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Thermostable Carboxylesterases from Hyperthermophiles
ChemInform, 2004AbstractFor Abstract see ChemInform Abstract in Full Text.
Atomi, H, Imanaka, T
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Enzymatic Activity of Human Carboxylesterases
Current Protocols in Toxicology, 2007AbstractThe carboxylesterases (CEs) are hydrolytic enzymes that metabolize xenobiotics that contain ester, thioester, or amide bonds. CEs are ubiquitously expressed but are found in highest concentration in membrane‐enriched fractions of the liver. This unit describes assays used to measure the enzymatic activity and tissue distribution of human CEs ...
Matthew K, Ross, Abdolsamad, Borazjani
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The carboxylesterases of Trypanosoma cruzi epimastigotes
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1987Carboxylesterase activity in Trypanosoma cruzi was found mainly in the microsomal (40%) and the cytosolic fraction (26%). The Vmax for p-nitrophenyl acetate was 28.50 and 17.60 nmol per min and mg of protein for the microsomal and the cytosolic fractions, respectively.
J, Aldunate +3 more
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Biochemistry, 1969
The molecular weight of pig liver carboxylesterase (~88% pure) has been determined as 163,000 (±15,000). The enzyme has two active sites per molecular weight of 163,000 as shown by titration with pnitrophenyl dimethylcarbamate and p-nitrophenyl diethyl phosphate.
Horgan, Douglas J. +4 more
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The molecular weight of pig liver carboxylesterase (~88% pure) has been determined as 163,000 (±15,000). The enzyme has two active sites per molecular weight of 163,000 as shown by titration with pnitrophenyl dimethylcarbamate and p-nitrophenyl diethyl phosphate.
Horgan, Douglas J. +4 more
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Journal of Biochemistry, 2010
A novel gene encoding an esterase from Geobacillus thermodenitrificans strain CMB-A2 was cloned, sequenced and functionally expressed in Escherichia coli M15. Sequence analysis revealed an open reading frame of 747 bp corresponding to a polypeptide of 249 amino acid residues (named EstGtA2).
David M, Charbonneau +2 more
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A novel gene encoding an esterase from Geobacillus thermodenitrificans strain CMB-A2 was cloned, sequenced and functionally expressed in Escherichia coli M15. Sequence analysis revealed an open reading frame of 747 bp corresponding to a polypeptide of 249 amino acid residues (named EstGtA2).
David M, Charbonneau +2 more
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Canadian Journal of Biochemistry, 1975
Chicken, sheep, and horse liver carboxylesterases have been purified by procedures involving ammonium sulfate fractionation, ion-exchange chromatography and gel filtration on Sephadex. The actual yields of the procedures described were as follows: chicken, 1 g from 2 kg of liver powder (chloroform–acetone); sheep, 200 mg from 400 g of powder ...
P A, Inkerman +5 more
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Chicken, sheep, and horse liver carboxylesterases have been purified by procedures involving ammonium sulfate fractionation, ion-exchange chromatography and gel filtration on Sephadex. The actual yields of the procedures described were as follows: chicken, 1 g from 2 kg of liver powder (chloroform–acetone); sheep, 200 mg from 400 g of powder ...
P A, Inkerman +5 more
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Drug Metabolism and Disposition, 2011
Human carboxylesterase (CES) 1 and CES2 are members of the serine hydrolase superfamily, and both exhibit broad substrate specificity and are involved in xenobiotic and endobiotic metabolism. Although expression of CES1 and CES2 occurs in several organs, their expression in liver and small intestine is predominantly attributed to CES1 and CES2 ...
Jie, Wang +4 more
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Human carboxylesterase (CES) 1 and CES2 are members of the serine hydrolase superfamily, and both exhibit broad substrate specificity and are involved in xenobiotic and endobiotic metabolism. Although expression of CES1 and CES2 occurs in several organs, their expression in liver and small intestine is predominantly attributed to CES1 and CES2 ...
Jie, Wang +4 more
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Biochemical and Biophysical Research Communications, 1975
During the final stage of purification of pig liver carboxylesterase on CM-Sephadex, several enzyme activities are present in addition to the pig liver carboxylesterase reported from this laboratory (Horgan et al., 1969a). Variation in substrate specificity and specific activity of the material isolated from CM-Sephadex has been analysed with four ...
Hamilton S.E. +3 more
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During the final stage of purification of pig liver carboxylesterase on CM-Sephadex, several enzyme activities are present in addition to the pig liver carboxylesterase reported from this laboratory (Horgan et al., 1969a). Variation in substrate specificity and specific activity of the material isolated from CM-Sephadex has been analysed with four ...
Hamilton S.E. +3 more
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Heterogeneity of carboxylesterases in rat liver cells
Biochemical Pharmacology, 1992Rat liver cells were separated into parenchymal cells (PC), Kupffer cells (KC) and endothelial cells (EC). The distribution of carboxylesterases (EC 3.1.1.1) between these cell types was investigated by PAGE and chromatogenic substrate staining, and compared with the results for total liver preparation and individual isoenzymes isolated by ...
R, Gaustad, T, Berg, F, Fonnum
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Measurement of Carboxylesterase (
AbstractMeasurement of Carboxylesterase (CES) Activities (Masakiyo Hosokawa, Chiba University, Chiba, Japan and Tetsuo Satoh, Biomedical Research Institute, Chiba, Japan). Mammalian carboxyesterase (CES) efficiently catalyzes the hydrolysis of a variety of ester‐ and amide‐containing chemicals.
Masakiyo, Hosokawa, Tetsuo, Satoh
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