Morphological and phylogenetic analyses of an <i>Elongisporangium</i> species and two <i>Globisporangium</i> species (Peronosporales, Oomycota) from China. [PDF]
Chen Y, Xing Y, Yu J, Peng J, Chen J.
europepmc +1 more source
Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
Preflight Calibration and Performance Assessment of the Geostationary Interferometric Infrared Sounder (GIIRS) Onboard the FengYun-4B Satellite. [PDF]
Lee L +6 more
europepmc +1 more source
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Sutimlimab in Patients With Cold Agglutinin Disease (CAD): Results From a Managed Access Program. [PDF]
Frey SM +5 more
europepmc +1 more source
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Dosimetric impact of radiofrequency tumor treating field arrays on dose on surface and at depth. [PDF]
Ashmeg S +3 more
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Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
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We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Selection of highly stress-tolerant yeast strains relevant for bioethanol fermentation using predictive growth models. [PDF]
Canseco Grellet MA +5 more
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