Results 51 to 60 of about 2,334 (253)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Aftercare Practitioners Facilitating the Recognition of Care-Experienced Youth in Finland

Nordisk Välfärdsforskning
While research has highlighted the challenges care leavers face, the understanding of the specific measures taken to support them remains limited. Practitioners’ actions in embodying the welfare system by implementing relevant laws and guidelines are ...
Johanna Hedman, Riikka Korkiamäki
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Empowering youth in care: the case of emancipatory research with care leavers

Journal de Ciencias Sociales
New sociology of childhood has emphasized that children are capable of being reflective, of behaving purposively and are able to influence the environment where they live. This concept is also referred to as ‘children as capable agents’ meaning that they
Caterina Arciprete, Mario Biggeri, Irene Fattacciu   +2 more
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

ALONE, LOST, AND UNPREPARED BY THE SYSTEM: INDIGENOUS CARE LEAVERS’ EXPERIENCES OF AGING OUT OF CHILD WELFARE CARE IN MANITOBA

International Journal of Child, Youth & Family Studies
Limited research has been carried out on Indigenous young people transitioning out of care in Canada. This article provides a coherent and meaningful account of the journey of aging out of care in the province of Manitoba, as presented by 17 Indigenous ...
Roberta L. Woodgate, Marlyn Bennett, Donna Martin, Pauline Tennent, Clayton Sandy, Shayna Plaut, Nicole Legras, Ashley Bell, Justin Lys   +8 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

New Professional Approaches for Care Leaver in Foster Care: Training “Coaching to Go!”

Lubelski Rocznik Pedagogiczny
Introduction: The number of young people with limited or non-existent social participation is growing. Characteristic is the proportion of young homeless individuals who had previous institutional contact with youth welfare.
Robert Frietsch, Dirk Holbach, Corinna Leißling   +2 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source