Results 101 to 110 of about 420,567 (309)

Insights into the human cDNA: A descriptive study using library screening in yeast

Journal of Genetic Engineering and Biotechnology
The utilization of human cDNA libraries in yeast genetic screens is an approach that has been used to identify novel gene functions and/or genetic and physical interaction partners through forward genetics using yeast two-hybrid (Y2H) and classical cDNA ...
Zina Alaswad, Nayera E. Attallah, Basma Aboalazm, Eman S. Elmeslhy, Asmaa S. Mekawy, Fatma A. Afify, Hesham K. Mahrous, Ashrakat Abdalla, Mai A. Rahmoon, Ahmed A. Mohamed, Ahmed H. Shata, Rana H. Mansour, Fareed Aboul-ela, Mohamed Elhadidy, Biola M. Javierre, Sherif F. El-Khamisy, Menattallah Elserafy   +16 more
doaj   +1 more source

cDNA library construction from a small amount of RNA: adaptor-ligation approach for two-round cRNA amplification using T7 and SP6 RNA polymerases

BioTechniques, 2005
In this study, we developed a method that allows cDNA library construction from a small amount of RNA without causing serious size bias in the resulting cDNA population.
Reiko Ohara, Reiko F. Kikuno, Hiroshi Kitamura, Osamu Ohara   +3 more
doaj   +1 more source

The Molecular Composition of Peptide Toxins in the Venom of Spider Lycosa coelestis as Revealed by cDNA Library and Transcriptomic Sequencing. [PDF]

Toxins (Basel), 2023
Wu X, Chen Y, Liu H, Kong X, Liang X, Zhang Y, Tang C, Liu Z.   +7 more
europepmc   +1 more source

Buoyancy regulation and aggregate formation in Amoebobacter purpureus from Mahoney lake [PDF]

, 1992
The meromictic Mahoney Lake (British Columbia, Canada) contains an extremely dense layer of purple sulfur bacteria (Amoebobacter purpureus). The buoyant density of Amoebobacter cells grown in pure culture at saturating light intensity was significantly ...
Bar-Ness, Bar-Or, Booker, Bryant, Bührer, Caldwell, Cline, Courtney, Dahlbäck, Dietrich, Doyle, Eichler, Fattom, Hart, Hartree, Herbert, Huheey, Hutchinson, Jenkinson, Jörg Overmann, Kjelleberg, Lehninger, Marshall, Mas, Nikovskaya, Norbert Pfennig, Northcote, Northcote, Northcote, Ofek, Oliver, Oliver, Overmann, Overmann, Overmann, Parker, Pfennig, Reed, Rosenberg, Siefert, Takakuwa, Tilzer, Walsby, Walsby   +43 more
core   +1 more source

Biomimetic Copper‐Doped Nano‐Aluminum Adjuvant Potentiates Therapy in Chemoresistant Acute Myeloid Leukemia

Advanced Healthcare Materials, EarlyView.
We developed a novel copper‐doped aluminum nano‐adjuvant (CuNA) to overcome cytarabine resistance in acute myeloid leukemia (AML). CuNA effectively sensitizes drug‐resistant AML cells to cytarabine by inducing mitochondrial dysfunction and inhibiting HMGCR/GPX4 to amplify ferroptosis.
Chao He, Lingxiao Zhang, Yue Xiong, Yu Wang, Yuqing Zeng, Dandan Su, Ran Wang, Ying Li, Hongbo Chen, Fang Cheng, Zhi Ping Xu   +10 more
wiley   +1 more source

In vivo gain-of-function cDNA library screening for colonization genes in a mouse model of pulmonary metastasis. [PDF]

STAR Protoc, 2022
Tu Z, Karnoub AE.
europepmc   +1 more source

A Modular Bioinstructive Platform Reveals Mechanistic Insights into Additive‐Free, Topography‐Driven Osteogenesis

Advanced Healthcare Materials, EarlyView.
Engineered microparticle topographies direct human mesenchymal stem cell osteogenesis without biochemical additives. This osteogenic commitment is driven by canonical Hedgehog signaling and followed by temporal IGF‐II engagement. Two‐photon polymerization demonstrates spatial control, enabling the engineering of topographical gradients that pattern ...
Fatmah I. Ghuloum, Leo A. H. Zeef, Lee A. Stevens, Marco A. N. Domingos, Susan J. Kimber, Mahetab H. Amer   +5 more
wiley   +1 more source

SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones

BMC Genomics, 2004
Background cDNA libraries are widely used to identify genes and splice variants, and as a physical resource for full-length clones. Conventionally-generated cDNA libraries contain a high percentage of 5'-truncated clones.
Poustka Annemarie, Schupp Ingo, Wellenreuther Ruth, Wiemann Stefan   +3 more
doaj   +1 more source

Molecular Diversity of Peptide Toxins in the Venom of Spider Heteropoda pingtungensis as Revealed by cDNA Library and Transcriptome Sequencing Analysis. [PDF]

Toxins (Basel), 2022
Liao Q, Kong X, Luo G, Wu X, Li Y, Liu Q, Tang C, Liu Z.   +7 more
europepmc   +1 more source

Counting absolute number of molecules using unique molecular identifiers [PDF]

, 2011
Advances in molecular biology have made it easy to identify different DNA or RNA species and to copy them. Identification of nucleic acid species can be accomplished by reading the DNA sequence; currently millions of molecules can be sequenced in a ...
Anna Vä, Jussi Taipale, Kasper Karlsson, Martin Bonke, Sten Linnarsson, Teemu Kivioja   +5 more
core   +1 more source