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Digital microfluidics for cell-based assays
Lab on a Chip, 2008We introduce a new method for implementing cell-based assays. The method is based on digital microfluidics (DMF) which is used to actuate nanolitre droplets of reagents and cells on a planar array of electrodes. We demonstrate that this method is advantageous for cell-based assays because of automated manipulation of multiple reagents in addition to ...
Irena, Barbulovic-Nad +3 more
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Metalloprotease inhibitor profiles of human ADAM8 in vitro and in cell-based assays
Biological chemistry, 2019ADAM8 as a membrane-anchored metalloproteinase-disintegrin is upregulated under pathological conditions such as inflammation and cancer. As active sheddase, ADAM8 can cleave several membrane proteins, among them the low-affinity receptor FcεRII CD23 ...
Uwe Schlomann +5 more
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Cell-based assays: fuelling drug discovery
Analytical and Bioanalytical Chemistry, 2010It has been estimated that over a billion dollars in resources can be consumed to obtain clinical approval, and only a few new chemical entities are approved by the US Food and Drug Administration (FDA) each year. Therefore it is of utmost importance to obtain the maximum amount of information about biological activity, toxicological profile ...
MICHELINI, ELISA +4 more
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Cell-Based Bioluminescence Screening Assays
2013Drug screening is an essential and widely used technique for drug discovery in various biomedical fields notably in oncology. Here we describe a functional screening assay based on the bioluminescence detection of a secreted luciferase for monitoring cell viability of cancer cells in a high-throughput format.
Romain J, Amante, Christian E, Badr
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2005
This chapter describes methods and tools for processing and visualizing data from high-throughput cell-based assays. Such assays are used to examine the contribution of genes to a biological process or phenotype (Carpenter and Sabatini, 2004). In principle, this can be done for any gene or combination of genes and for any biological process of interest.
W. Huber, F. Hahne
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This chapter describes methods and tools for processing and visualizing data from high-throughput cell-based assays. Such assays are used to examine the contribution of genes to a biological process or phenotype (Carpenter and Sabatini, 2004). In principle, this can be done for any gene or combination of genes and for any biological process of interest.
W. Huber, F. Hahne
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Context-Enriched Cell-Based Assays
Genetic Engineering & Biotechnology News, 2016openaire +2 more sources
Advances in cell-based permeability assays to screen drugs for intestinal absorption
Expert Opinion on Drug Discovery, 2020Introduction: Successful oral therapy requires sufficient intestinal absorption to enable the drug to reach its site of action. Evaluation of intestinal permeability is important for candidate selection during drug discovery and development.
D. Volpe
semanticscholar +1 more source
Cell Based Biological Assay Using Microfluidics
2008A microfluidic device has been designed to measure and manipulate microdroplets, in which protein expression is induced in single cells. The device exploits the permeation of water through poly (dimethylsiloxane) (PDMS) in order to keep the concentration of solutes in aqueous picoliter volume microdroplets stored in wells.
Shim +5 more
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Label-Free Cell-Based Functional Assays
Combinatorial Chemistry & High Throughput Screening, 2008Label-free technologies based on electrical impedance or refractive index are new tools for measuring a cell-based functional response. Although the technologies are relatively new to high throughput screening cell-based applications, they are rapidly generating interest in that they are able to measure a phenotypic response using cells natively ...
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