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Separation of mother and daughter cells
2002Publisher Summary The budding yeast Saccharomyces cerevisiae ( S. cerevisiae ) divides asymmetrically. In vegetative growth, yeast cells reproduce by budding, and the position where the bud forms ultimately determines the plane of cell division. This chapter describes the detailed procedures for the separation and isolation of mothers and daughters.
Peter U, Park +2 more
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1998
Abstract The successful separation of specific cells requires not only a knowledge of potential methods, but also detailed protocols that are known to work well in practice. This book is unique in that it brings together the best methods currently used for separating cells.
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Abstract The successful separation of specific cells requires not only a knowledge of potential methods, but also detailed protocols that are known to work well in practice. This book is unique in that it brings together the best methods currently used for separating cells.
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Separation of White Blood Cells
Nature, 1964A CONVENIENT way of obtaining white cells from whole blood is simply to allow EDTA-blood to settle in siliconized glasses and then pipette off the leucocyte-rich supernatant after 1.5 h or more. A more rapid separation occurs when agents which aggregate erythrocytes are added to the blood1. The effect of these agents may be utilized even without mixing
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Cell Separations by Flow Cytometry
2003Flow cytometry is a means of measuring the physical and chemical characteristics of particles in a fluid stream as they pass one by one past a sensing point. The modern flow cytometer consists of a light source, collection optics and detectors, and a computer to translate signals into data.
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WNK kinases sense molecular crowding and rescue cell volume via phase separation
Cell, 2022Arohan R Subramanya
exaly
Separation of cell layers in hydra
Wilhelm Roux' Archiv f�r Entwicklungsmechanik der Organismen, 1961The mesolamella of hydra is dissociable by means of divalent metal chelation with EDTA and is more susceptible to this treatment than the intercellular cements of each layer. Uncontaminated gastrodermal and epidermal cells may be obtained by this treatment.
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Basic Principles and Recent Advances in Magnetic Cell Separation
Magnetochemistry, 2022Marie Frénéa-Robin, Julien Marchalot
exaly