Results 121 to 130 of about 6,520,444 (304)

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

ELISPOT assay to measure antigen-specific murine CD8(+) T cell responses.

, 2001
The enzyme-linked immunospot technique (ELISPOT) relies on the visualization of cytokine secretion by individual T cells following in vitro stimulation with antigen. This assay has been developed and standardized for the quantitative detection of antigen-
Luzia H Carvalho, Carvalho, LH, Fidel Zavala, Julius C.R Hafalla, Hafalla, JC, Zavala, F   +5 more
core   +1 more source

Protocol for mapping T cell activation using single-cell RNA-seq

STAR Protocols
Summary: Stimulation of CD4 T cells with anti-CD3/CD28 is a commonly used model to study T cell activation. Here, we present a protocol for investigating T cell activation based on anti-CD3/CD28 bead stimulation and single-cell RNA sequencing (scRNA-seq).
Hui Li, Yifei Liu, Xuefei Wang, Shiya Yu, Junliang Wang, Yue Hu, Ni Hong, Wenfei Jin   +7 more
doaj   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

A Microactuator Array Based on Ionic Electroactive Artificial Muscles for Cell Mechanical Stimulation

Biomimetics
Mechanical stimulation is prevalent within organisms, and appropriate regulation of such stimulation can significantly enhance cellular functions. Consequently, the in vitro construction and simulation of mechanical stimulation have emerged as a research
Jing Gu, Zixing Zhou, Yang Xie, Xiaobin Zhu, Guoyou Huang, Zuoqi Zhang   +5 more
doaj   +1 more source

Optomechanically Actuated Hydrogel Platform for Cell Stimulation with Spatial and Temporal Resolution. [PDF]

ACS Biomater Sci Eng, 2023
Ramey-Ward AN, Dong Y, Yang J, Ogasawara H, Bremer-Sai EC, Brazhkina O, Franck C, Davis M, Salaita K.   +8 more
europepmc   +1 more source

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

Impact of exogenous direct electric current on the expression of mRNA related to OPG, in SaOS-2 cells using quantitative reverse transcription polymerase chain reaction: A qualitative and quantitative analysis

Journal of Oral Biology and Craniofacial Research
Objective: To assess OPG gene expression in SaOS-2 cells after exposure to pulsed direct current (30 μA/10 s, square wave) using qRT-PCR at different time points (5, 7,12, and 24 h).
Aparajita Pandey, Ashish Agrawal, Parimal Das, Ritu Dixit, Prashant Ranjan, Sushit kumar sonu   +5 more
doaj   +1 more source

Dicofol Stimulation of Cell Proliferation [PDF]

Bulletin of Environmental Contamination and Toxicology, 2001
K, Du, X, Xu
openaire   +2 more sources