Results 91 to 100 of about 10,829,612 (291)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Charge carrier separation induced by intrinsic surface strain in pristine ZnO nanowires

, 2010
We predict by first-principles calculations a spontaneous charge carrier separation mechanism in pristine [0001]-oriented ZnO nanowires. We find that the shrinking strain induced by surface reconstruction causes electrons and holes to separate and move ...
Li, Chun, Chen, Changfeng, Chun Li, Zi-Yue Zhang, Changfeng Chen, Guo, Wanlin, Liangzhi Kou, Kou, Liangzhi, Zhang, Zi-Yue, Wanlin Guo   +9 more
core   +1 more source

Internalization of therapeutic antibodies into dendritic cells as a risk factor for immunogenicity

Frontiers in Immunology
IntroductionImmunogenicity, the unwanted immune response triggered by therapeutic antibodies, poses significant challenges in biotherapeutic development.
Michel Siegel, Anna-Lena Bolender, Axel Ducret, Johannes Fraidling, Katharina Hartman, Cary M. Looney, Olivier Rohr, Olivier Rohr, Timothy P. Hickling, Hubert Kettenberger, Martin Lechmann, Céline Marban-Doran, Thomas E. Kraft   +12 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Surface-Charge-Driven Ferroptosis and Mitochondrial Dysfunction Is Involved in Toxicity Diversity in the Marine Bivalve Exposed to Nanoplastics

Nanoplastics (NPs) pervade daily life, posing serious threats to marine ecosystems. Despite the crucial role that surface charge plays in NP effects, there is a substantial gap in our understanding of how surface charge influences NP toxicity. Herein, by
Hao Jiang (95017), Xiao-Xia Zhou (1461487), Wenzhi Liu (3997184), Yanfei Zhou (704684), Jorge L. Gardea-Torresdey (1455463), Fengyuan Chen (771754), Yanping Li (90163), Bing Yan (170259)   +7 more
core   +2 more sources

Measurement of surface potential decay of corona-charged polymer films using the pulsed electroacoustic method

, 2007
In this paper, the pulsed electroacoustic (PEA) technique that allows the determination of space charge in a dielectric material has been used to monitor the electrical potential decay of corona-charged polyethylene films of different thicknesses.
Xu, Z, Chen, G, Zhang, L W
core   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Near-Field Analysis of Terahertz Pulse Generation From Photo-Excited Charge Density Gradients [PDF]

, 2015
Excitation of photo-current transients at semiconductor surfaces by subpicosecond optical pulses gives rise to emission of electromagnetic pulses of terahertz (THz) frequency radiation.
Natrella, M, Freeman, JR, Renaud, CC, Graham, CS, Linfield, EH, Moodie, DG, Cannard, PJ, Davies, AG, Mitrofanov, O, Seeds, AJ, Mueckstein, R, Hatem, O, Robertson, MJ   +12 more
core   +1 more source

Recent insights into the molecular mechanism of ubiquinol oxidation by cytochrome bc1

FEBS Open Bio, EarlyView.
The review reflects on the mechanism of the catalytic reaction in cytochrome bc1: the electron bifurcation that involves the separation of two electrons derived from the quinol oxidation to opposite sides of the enzyme across the membrane. This review summarizes the long‐standing effort to understand the mechanism of quinol oxidation catalyzed by ...
Anna Wójcik‐Augustyn, Artur Osyczka, Marcin Sarewicz   +2 more
wiley   +1 more source