Results 41 to 50 of about 10,829,612 (291)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Zwitterionic Osmolytes Employ Dual Mechanisms for Resurrection of Surface Charge under Salt-stress [PDF]
, 2022 Salt imbalance in cells is a major detrimental abiotic stress which causes ionic toxicity and disrupts important cellular functions. To counter this saline stress, cell often produces low molecular weight cosolutes, known as osmolytes, which have the ...
T. N., Narayanan +3 more
core +2 more sources
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Effects of paclitaxel nanocrystals surface charge on cell internalization
European Journal of Pharmaceutical Sciences, 2016 In this study, we investigated the influence of paclitaxel nanocrystals (PTX-NC) surface charge on cell internalization and cell cytotoxicity. PTX-NCs were prepared using the nano-precipitation method. The surface-modified PTX-NCs were prepared using an absorption method with positively charged poly(allylamine hydrochloride) (PAH) and negatively ...
Jin-Seok Choi, Jeong-Sook Park
openaire +2 more sources
EQCM and EIS Study of the Effect of Potential of Zero Charge on Escherichia Coli Biofilm Development
International Journal of Electrochemical Science, 2010 Using EQCM method, the influence of electrode potential on Escherichia coli (E. coli) biofilm development was investigated in a range from −0.6V to 0.6V. Parabolic dependence of the cell density on the electrode potential was observed.
Xuan-Hui Xie, Erin Lin Li, Zi Kang Tang
doaj +1 more source
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
Emerging experimental and computational methods for studying redox‐regulated structural transitions
FEBS Letters, EarlyView.Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
Cell Transplantation, 1997 This article presents a novel, clinically relevant electrostatic endothelial cell transplantation (seeding/sodding) device (U.S. & Foreign Patent Protections Pending) for small-diameter (
Gary L. Bowlin, Stanley E. Rittgers
doaj +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Biophysical Properties of Blood Cells in People Aged 36–59 Years Under Mechanical Stress in vitro
Журнал медико-биологических исследованийThe influence of the adenosine triphosphate molecule on cell signalling cascades under different physiological conditions, such as disrupted regeneration processes in the body, is of particular interest for scientists. The purpose of this research was to
Evgeniya A. Sladkova +3 more
doaj +1 more source