Results 111 to 120 of about 6,074,414 (310)

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

open access: yesFEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell   +2 more
wiley   +1 more source

The Effect of Polyhydroxyethylmethacrylate (Poly-HEMA), as a Cell Culture Substrate, on Viable and Preserving Nature of Retinal Pigment Epithelium (RPE) Cells

open access: yesمجله دانشکده پزشکی اصفهان, 2014
Background: Retinal pigment epithelium (RPE) cells play an important role in the maintenance of normal function of the retina. Tyrosinase is a marker of RPE cells that functions in synthesis of melanin. Polymer of polyhydroxyethylmethacrylate (Poly-HEMA)
Fatemeh Nazem-Roaya   +6 more
doaj  

Purification of Lipid Rafts from Cultured Cells

open access: yesThe Scientific World Journal, 2002
Lipid-rich lipid rafts are microdomains of the plasma membrane that are resistant to low concentrations of nonionic detergent. This forms the basis for their isolation.
John Graham
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

open access: yesFEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav   +2 more
wiley   +1 more source

Platelet lysate as a serum substitute for 2D static and 3D perfusion culture of stromal vascular fraction cells from human adipose tissue [PDF]

open access: yes, 2009
Fetal bovine serum (FBS) and fibroblast growth factor (FGF)-2 are key supplements for the culture of stromal vascular fraction (SVF) cells from adipose tissue, both for typical monolayer (2D) expansion and for streamlined generation of osteogenic ...
Alini, Mauro   +8 more
core  

Prostate-regenerating capacity of cultured human adult prostate epithelial cells

open access: yes, 2010
Experimentation with the progenitor/stem cells in adult prostate epithelium can be inconvenient due to a tight time line from tissue acquisition to cell isolation and to downstream experiments.
Taylor, Renea A.   +9 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

Glycosylation of Chrysin by Cultured Cells of

open access: yesNatural Product Communications, 2016
The biotransformation of chrysin was investigated using cultured plant cells of Eucalyptus perriniana as biocatalysts. Chrysin was glucosylated to chrysin 7- O -β-D-glucoside and chrysin 7- O -β-gentiobioside.
Kei Shimoda   +3 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

open access: yesFEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil   +3 more
wiley   +1 more source

The release of lysosomal Pro⁃cathepsin L of BV⁃2 cells upon lipopolysaccharide stimulation and its toxicity to neurons

open access: yesChinese Journal of Contemporary Neurology and Neurosurgery, 2011
Objective To investigate the secretion of lysosomal Pro ⁃ cathepsin L and its contribution to neuronal apoptosis upon lipopolysaccharide (LPS) stimulation.
Hui ZHANG   +4 more
doaj  

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