Results 211 to 220 of about 20,820 (288)

Diagnostic investigations: harmless tests or booby traps? [PDF]

Diagnosis (Berl)
Elwyn G, Zwaan L, Hairwassers D, Han PKJ.   +3 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Public involvement in the design of a complex neurosurgical clinical trial (the GDNF trial) and an associated support package: reflections and recommendations. [PDF]

Trials
Bakshi N, Bale C, Binu T, Brown G, Calder J, Campbell R, George H, Gosden L, Hobbs A, Phipps T, Prettyman R, Roach A, Bodicoat DH, Whone A.   +13 more
europepmc   +1 more source

"Web, censures et contournements des censures", séminaire

PandHeMic, 2018
openaire   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Dream Patterns in Patients with Acute Myocardial Infarction: Data from the STEP-IN-AMI Trial. [PDF]

J Clin Med
Roncella A, Pasceri V, Pristipino C, Di Michele L, Irini D, Allan R, Pelliccia F, Speciale G.   +7 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Professional Pearls. [PDF]

J Gen Intern Med
Menzin ER.
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Shame among medical learners: An uncertainty-focused conception. [PDF]

Patient Educ Couns
Han PKJ, Schaufel M, Schei E.
europepmc   +1 more source