Results 81 to 90 of about 6,573 (256)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Censure: Moral and Sociological [PDF]

, 2017
The concept of censure, as developed in different ways, in the work of Sumner and von Hirsch, has enormous potential—though each of the accounts has its own limitations—In this chapter, I discuss both theories and show some way in which there is a common ground between them, and to offer some suggestions as to ways that this common ground might be ...
openaire   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Identification of the plant mitochondrial OrfX protein: A mass spectrometry approach

FEBS Letters, EarlyView.
The mitochondrial genome of plants contains an open reading frame, orfx, which encodes a rare protein that has so far escaped mass spectrometric detection. The protein resembles the c‐subunit of bacterial twin‐arginine‐motif‐dependent protein translocases (TatC).
Matthias Döring, Jan de Jonge, Hans‐Peter Braun, Nils Rugen   +3 more
wiley   +1 more source

Un’altra visione delle cose: note sulla satira nel Risorgimento

Diacronie. Studi di Storia Contemporanea, 2012
The paper draws a historical portrait of literary satire in Italy from 1830 up to the achievement of Italian unification. Through four paragraphs, I will discuss Carlo Bini’s gloomy reinterpretation of Sterne’s style; Giacomo Leopardi’s Neapolitan ...
Marco Viscardi (1978) si è addottorato in Filologia Moderna nel 2009 (Università degli Studi di Napoli “Federico II”) discutendo una tesi sulle lezioni dantesche di F. A. Ozanam (1813-1852); attualmente borsista presso l’Istituto Italiano per gli Studi Storici, lavora ad una monografia su satira ed umorismo in Italia fra 1816 e 1861. Cultore della materia presso le cattedre di Storia della Critica Letteraria e Letteratura Italiana II, titolare il prof. Giovanni Maffei, si è occupato di romanzo storico fra Otto e Novecento e di storiografia letteraria, e ha pubblicato interventi su Tomasi di Lampedusa, Manganelli e Giuseppe Giusti.
doaj  

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

La lettre comme moyen de dénonciation : étude de « Pour la liberté de pensée »de Voltaire

Revista de Lenguas Modernas, 2010
Dans l’une de ces nombreuses lettres intitulée Pour la liberté de pensée nous étudierons les raisons pour lesquelles Voltaire considère qu’il ne faut pas interdire les livres. Pour ce faire, nous analyserons les outils linguistiques utilisés par l’auteur
Beatriz Rodríguez Oconitrillo
doaj