Results 31 to 40 of about 174,361,874 (315)

Extracellular vesicle lipidomics liquid biopsy reveals systemic and tumor microenvironment metabolic reprogramming in ovarian cancer

open access: yesMolecular Oncology, EarlyView.
Extracellular vesicle (EV) lipidomic profiling of plasma and ascites from patients with high‐grade serous ovarian cancer and of ovarian cancer cell lines reveals enrichment of triglycerides (TGs), diacylglycerols (DGs), phosphatidylcholine (PCs), sphingomyelins (SMs), reflecting tumor metabolic reprogramming.
Shikha Rani   +9 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Resumming spinning black hole dynamics at third post-Minkowskian order

open access: yesJournal of High Energy Physics
We investigate the relativistic scattering of spinning black holes using modern amplitude methods within a heavy-mass effective field theory formalism at third post-Minkowskian order.
N. E. J. Bjerrum-Bohr   +3 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Perturbations of plane waves and quadratic quasinormal modes on the lightring

open access: yesJournal of High Energy Physics
We study second order gravitational perturbations on plane wave spacetimes from both the metric and curvature perturbation points of view. For the former, we explicitly use the isometries of the background to introduce tensor oscillator harmonics, which ...
Kwinten Fransen   +2 more
doaj   +1 more source

Air pollution in China: Spatial patterns and spatial coupling with population and economy

open access: yesFrontiers in Environmental Science, 2022
This paper analyzes the daily data of China’s air quality index (AQI) from 2015 to 2020 using kriging, spatial autocorrelation, and gravity center method. The results confirm that China’s air pollution is gradually controlled. From 2015 to 2020, the AQI,
Dachuan Yan   +4 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

open access: yesFEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer   +7 more
wiley   +1 more source

A Method for Extracting a Laser Center Line Based on an Improved Grayscale Center of Gravity Method: Application on the 3D Reconstruction of Battery Film Defects

open access: yesApplied Sciences, 2023
Extraction of the laser fringe center line is a key step in the 3D reconstruction of linear structured light, the accuracy of which is directly related to the quality of the 3D model. A laser center line extraction method based on an improved gray center
Rongbin Yao   +6 more
doaj   +1 more source

100 Glimpses : Lyle Kirk

open access: yes, 2023
100 Glimpses is a series of short documentaries produced by the Sight Center of Northwest Ohio. The series explores the organization's history. The Sight Center was founded in 1923 as the Toledo Society for the Blind.
Sight Center of Northwest Ohio (Toledo, Ohio)
core  

Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora

open access: yesFEBS Open Bio, EarlyView.
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi   +6 more
wiley   +1 more source

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