An interpretable machine learning model integrating mucosal and cellular immunity to predict SARS-CoV-2 reinfection: a multicenter cohort study. [PDF]
Ran J +14 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
South Africa [cartographic material] : mining activity and production.
"800479 (543001) 3-86."; Includes graph.; Also available online http://nla.gov.au/nla.map ...
United States. Central Intelligence Agency.
core
Linking formulation composition to pharmacokinetic and pharmacodynamic responses: a predictive framework for sustained-release tablets. [PDF]
Song S +5 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Homecoming parade float; Phi Beta float; "Central Will Star"; one female student sitting on float
Homecoming parade float built by Phi Beta float carries a female student passenger in costume through Fayette, Mo. The float reads, "Central Will Star".
Central Methodist University, Smiley Memorial Library
core
Fuzzy topological analysis of fuzzy Helm graphs with applications to protein interaction networks. [PDF]
Mufti ZS +3 more
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Graph-Calibrated Differential Privacy for Correlated IoT Sensing Streams. [PDF]
Chen Z, Chen J.
europepmc +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source

