Results 41 to 50 of about 371,811 (269)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Myogenic Fusogen‐Engineered Lipid Nanoparticles Enhance mRNA Delivery in Skeletal Muscle

open access: yesAdvanced Functional Materials, EarlyView.
This study reports a biomimetic strategy of engineering full‐length Myomaker, a muscle‐specific fusogen, into lipid nanoparticles (LNPs) to harness the native myoblast fusion capability for skeletal muscle mRNA delivery. The resulting Mymk‐LNPs enhance transfection in differentiating myocytes and enable Cre‐mediated reporter activation in injured ...
Fangyu Zhang   +18 more
wiley   +1 more source

Microfluidic Nano‐Assembly of Red‐Blood‐Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles

open access: yesAdvanced Healthcare Materials, EarlyView.
Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj   +23 more
wiley   +1 more source

Efficiency Analysis of the 946–P-1–B Centrifugal Pump in PT Installation and Shipment Area. Pertamina Refinery Unit II Production Sungai Pakning

open access: yesMetal: Jurnal Sistem Mekanik dan Termal
Centrifugal pumps are a type of pump that is widely used in various industrial applications to convey liquids. This article presents a comprehensive review of centrifugal pumps, including working principles, main components, pump power, and pump ...
Ridwan Abdurrahman   +1 more
doaj   +1 more source

Internal Flow Characteristics of High-Specific-Speed Centrifugal Pumps with Different Number of Impeller Blades under Large Flow Conditions

open access: yesMachines, 2023
As compared with a conventional centrifugal pump, a high-specific-speed centrifugal pump mostly operates under large flow conditions. In this paper, a typical high-specific-speed centrifugal pump is examined, and the effect of the blade number on the ...
Chuan Wang   +6 more
doaj   +1 more source

Ionizable Lipid‐Dependent Optimization of Steroid Lipid Nanoparticles With Tunable Immunomodulatory Properties

open access: yesAdvanced Materials, EarlyView.
A novel engineering strategy that establishes material design principles for incorporating anti‐inflammatory steroids into lipid nanoparticles to reduce LNP‐induced inflammation while retaining mRNA delivery. Results are validated in vitro and in three animal models of inflammation and autoimmunity in vivo.
Ajay S. Thatte   +21 more
wiley   +1 more source

Pump Combination Regulating Method of Centrifugal Water Injection Station for Energy Saving

open access: yesShiyou jixie, 2019
Most researches on pump station optimization focus on the pump station operation parameters, which gives the start and stop situation under the minimum power.
Cui Yuhai   +6 more
doaj  

The influence of blade curvature radius on the internal unsteady characteristics of a centrifugal pump

open access: yesEnergy Science & Engineering
To study the influence of the blade curvature radius on the flow‐induced vibration and noise of the centrifugal pump, the internal sound field of the IS 80‐65‐160 centrifugal pump was numerically calculated by adjusting the blade wrap angles to change ...
Yuqin Wang, Shuai Liu, Zhenhu Cao
doaj   +1 more source

Numerical Simulation Research on Radial Force of Centrifugal Pump with Guide Vanes

open access: yesShock and Vibration, 2021
To reveal the internal unsteady flow state of the guide vane centrifugal pump, in this paper, the standard SST k‐ω turbulent flow model is used for unsteady numerical simulation of the centrifugal pump.
Dongrong Meng   +3 more
doaj   +1 more source

Programmable Functional Silicification of DNA Origami Nanostructures

open access: yesAdvanced Materials, EarlyView.
This work introduces additional functionality into silica‐coated DNA origami nanostructures using non‐standard silica precursors. A fluorescent precursor enables enhanced intracellular tracking, while a disulfide‐containing reagent yields redox‐responsive, degradable silica coatings.
Anna V. Baptist   +4 more
wiley   +1 more source

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