Results 161 to 170 of about 208,778 (199)
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Computer Simulation of Density-Gradient Centrifugation
1979Modern biochemical techniques are continuously refined to produce new and improved results. Improvements in the useful power of a biochemical technique can in principle be achieved in two ways. One is the classic trial-and-error approach whereby the parameters of the experimental conditions are varied one by one until a suitable set of conditions is ...
Jens Steensgaard, Niels Møller
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Density Gradient Centrifugation of a Murine Leukemia Virus
Science, 1964The Rauscher leukemia virus separated as a single band upon density gradient centrifugation in cesium chloride, rubidium chloride, sucrose, potassium citrate, or potassium tartrate. Prolonged exposure to concentrated potassium citrate or potassium tartrate solutions caused lysis of the virus; the resulting products, having different densities ...
O'conner, T E +2 more
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Use of density gradients in a disk centrifuge
Journal of Colloid and Interface Science, 1986Abstract Conditions were examined under which stable sedimentation occurs in a disk centrifuge. Temperature gradients, largely caused by evaporative cooling at the spin-fluid meniscus, appeared to be the primary cause for convective streaming and its deleterious effects in the determination of particle size distributions.
Hans Coll, Charles G Searles
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An Analysis of Fish Genomes by Density Gradient Centrifugation
European Journal of Biochemistry, 1980DNA was prepared from 33 species of fishes representing 12 of the 31 orders of Teleostei and one order of Chondrichthyes. DNA samples were investigated to determine modal and mean buoyant densities in CsCl (q0 and 〈q〉), main‐band asymmetry, intermolecular compositional heterogeneity and base composition.
Audrey E. V. Haschemeyer +4 more
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Density gradient centrifugation of a graft copolymer
Journal of Polymer Science Part A: General Papers, 1964AbstractA well‐defined graft copolymer of polystyrene and cellulose acetate has been investigated using the density gradient ultracentrifugation method. The initial sample showed two peaks. These were found to be pure graft copolymer and polystyrene; the latter was easily removed by further extraction of the mixture with benzene. The composition of the
V. Stannett, H. A. Ende
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Separation of cytoplasmic particles by centrifugation in a density-gradient
Experientia, 1953Erste Versuche zur Ultrazentrifugierung isolierter Zytoplasmapartikelfraktionen aus Krallenfroschleber (Xenopus laevis Daud.) in «Diodon»-Saccharose-Gradienten mit verschiedenen Dichtebereichen zeigen, dass mitochondrien- und mikrosomenartige Zellpartikel in verschiedenen Zonen der Gradienten ins Dichtegleichgewicht gebracht werden konnen. Die Lage der
M. Ottesen, H. Holter, R. Weber
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Centrifugation of Liver Microsomes on Metrizamide Density Gradients
Preparative Biochemistry, 1976Abstract Metrizamide, 2-(3-acetamido-5-N-methyl-acetamido-2, 4, 6–triiodobenzamido)-2-deoxy-D-glucose (mol. wt 789), inhibits liver microsomal enzymes only to a small degree and it has no solubilization or detergent effects on the membrane. Four hour centrifugation in a continuous metrizamide gradient is sufficient for microsomal vesicles to attain ...
Hans Fehrnström +2 more
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Zonal separations by density-gradient centrifugation
Archives of Biochemistry and Biophysics, 1953Summary Zonal separations in the centrifuge have been obtained by floating a layer of a solution of particles on a second solution which has a density gradient and in which the particles are soluble. During subsequent centrifugation all those particles of the same size, shape, and density sedimented as a zone.
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Density gradient centrifugation in angle-head rotors
Analytical Biochemistry, 1964Abstract By utilizing DNA and T3 bacteriophage as test materials, good isopyenic banding in CsCl was obtained in #30 and #40 Spinco fixed-angle rotors. Values for the buoyant densities of the materials tested agree with published values and density gradients formed after prolonged centrifugation approximate the calculated compositional gradient at ...
G.B. Cline, N.G. Anderson, W.D. Fisher
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Differentiation of reoviruses and picornaviruses by density gradient centrifugation
Archiv f�r die gesamte Virusforschung, 1967The two HA moieties of reovirus (infectious particle and top component) were recentrifuged in a CsCl gradient. They were stable under these conditions and purified to such an extent that the top component HA moiety contained only 4 TCD50 per unit. Mixtures of poliovirus and reovirus (all three prototypes) were centrifuged in a CsCl density gradient ...
M. Taher, Reto Engler, A. Fouad
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