Results 191 to 200 of about 246,524 (265)

Comprehensive Protocol for Handling Human Small Airway Epithelial Cells (HSAECs) to Establish Air-Liquid Interface (ALI) Cultures With TEER-Based Barrier Integrity Assessment. [PDF]

Bio Protoc
Jakubczyk D, Pyclik M, Kozakiewicz D, Macała J, Zabłocka A, Górska S.   +5 more
europepmc   +1 more source

Patient therapy outcome modeling in cancer organoids is improved by cancer‐associated fibroblasts and organoid assembly convolution

Molecular Oncology, EarlyView.
Patient‐derived organoids (PDOs) from pancreatic, colorectal, and gastric cancers were used to evaluate standard and experimental therapies. Incorporating cancer‐associated fibroblasts (CAFs) into organoid cultures improved patient therapy outcome prediction.
Marcin Grochowski, Liudmyla Dolinchuk, Michał Jerzak, Albert Gandurski, Tomasz Grochowski, Weronika Wojtyś, Maciej Zadrożny, Wojciech Kaźmierczak, Małgorzata Lenarcik, Marta Matejak‐Górska, Radosław Samsel, Tomasz Olesiński, Dawid Walerych   +12 more
wiley   +1 more source

In-Culture Antibody Capture Using Transient CHO Expression Systems. [PDF]

Bio Protoc
Zhou J, Gebhardt L, Abel M, Vogt A, Shin BH, Santos A, Puginier J, Wurm FM, Wurm MJ, Herrick-Wagman SL, Adeniyi A, Yan GG, Lim S, Lin L, D'Antona AM, Zhong X.   +15 more
europepmc   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

A Suspension-Trapping Protocol for Bottom-Up Proteomics Sample Preparation. [PDF]

Bio Protoc
Schrader J, Province D, DaSilva NA, Liu C.   +3 more
europepmc   +1 more source

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source

Evaluating Thioredoxin-Mediated CF_oCF₁ Reduction Using an In Vitro Thylakoid Assay. [PDF]

Bio Protoc
Sekiguchi T, Yoshida K, Hisabori T.
europepmc   +1 more source

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

Protocol for the biochemical isolation of endosomal membranes associated with endoplasmic reticulum in HeLa cells. [PDF]

STAR Protoc
Da Graça J, Morel E.
europepmc   +1 more source

Identification of the Subcompartment-Specific Mitochondrial Proteome by APEX2 Proximity Labeling in <i>Saccharomyces cerevisiae</i>. [PDF]

Bio Protoc
Spänle L, Herrmann JM.
europepmc   +1 more source