Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Protocol for first-hit oncogenic organoid modeling and lentiviral functional screening. [PDF]
STAR ProtocYousef F +4 more
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Development and evaluation of a novel experimental autoimmune prostatitis model induced by a prostate-specific antigen mixture in rats. [PDF]
Biochem Biophys RepHong X +7 more
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Protocol for generating human iPSC-derived cardiomyocytes using VCAM1-based sorting and micropatterned culture. [PDF]
STAR ProtocFurtado M +4 more
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Protocol for high-throughput droplet-engineered TIL-retaining tumor organoids in triple-negative breast cancer. [PDF]
STAR ProtocZhou X +6 more
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Experimental Study on Anxiolytic and Antidepressant Effects of Zhi-Chi-Jie-Yu Granules in CUMS Mice and Prediction of Underlying Molecular Mechanisms. [PDF]
Neuropsychiatr Dis TreatLuo Y +6 more
europepmc +1 more source
Protocol for multimodal programming of murine macrophage phenotypes. [PDF]
STAR ProtocMirji G, Bhat SA, Shinde RS.
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Synergistic eradication of NDM-1 <i>Klebsiella pneumoniae</i> biofilm infection by a Meropenem/EDTA Co-delivery system based on gelatin microspheres. [PDF]
Mater Today BioChu Z +9 more
europepmc +1 more source