Results 121 to 130 of about 7,143,717 (276)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

The RED scheme: Rate-constant estimation from pre-steady state weighted ensemble simulations. [PDF]

J Chem Phys, 2021
DeGrave AJ, Bogetti AT, Chong LT.
europepmc   +1 more source

Stochastic processes in chemical physics

, 2009
The Advances in Chemical Physics series provides the chemical physics and physical chemistry fields with a forum for critical, authoritative evaluations of advances in every area of the discipline. Filled with cutting-edge research reported in a cohesive
Shuler, K E
core  

Structures and electronic properties of B3Sin- (n=4-10) clusters: A combined ab initio and experimental study

, 2017
The anionic silicon clusters doped with three boron atoms, B3Sin- (n = 4-10), have been generated by laser vaporization and investigated by anion photoelectron spectroscopy. The vertical detachment energies (VDEs) and adiabatic detachment energies (ADEs)
Qin, Ying, King, R. Bruce, R. Bruce King, Maodu Chen, Chen, Maodu, Xiaoqing Liang, Zhao, Jijun, Lu, Sheng-Jie, Xu, Hong-Guang, Liang, Xiaoqing, Zheng, Weijun, Wu, Xue, Sheng-Jie Lu, Xiaoming Huang, Hong-Guang Xu, Ying Qin, Huang, Xiaoming, Jijun Zhao, Weijun Zheng, Xue Wu   +19 more
core   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Visualizing the Intellectual Structure of Iranian Physicists in Scisearch during 1990-2009: An Author Co-Citation Analysis (ACA)

International Journal of Information Science and Management, 2012
The purpose of this paper was to study and map the intellectual structure of Iranian physicists using author co-citation technique during 1990-2009.
F. Osareh, R. Khademi
doaj  

Concurrence of directional Kondo transport and incommensurate magnetic order in the layered material AgCrSe2

Communications Physics
In this work, we report on the concurrent emergence of the directional Kondo behavior and incommensurate magnetic ordering in a layered material. We employ temperature- and magnetic field-dependent resistivity measurements, susceptibility measurements ...
José Guimarães, Dorsa S. Fartab, Michal Moravec, Marcus Schmidt, Michael Baenitz, Burkhard Schmidt, Haijing Zhang   +6 more
doaj   +1 more source

Physics-constrained machine learning for reduced composition space chemical kinetics

Data-Centric Engineering
Modeling detailed chemical kinetics is a primary challenge in combustion simulations. We present a novel framework to enforce physical constraints, specifically total mass and elemental conservation, during the reaction of ML models’ training for the ...
Anuj Kumar, Tarek Echekki
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source