Results 111 to 120 of about 15,961 (231)

Temperature Interference on ZIKV and CHIKV Cycles in Mosquitoes and Mammalian Cells

Pathogens
Temperature is a determining factor for the viral cycle. In this study, we investigate the effect of different temperatures on the cycles of two important arboviruses—Zika (ZIKV) and Chikungunya (CHIKV)—in Vero (mammalian) and C6/36 (mosquito) cells. We compare genome quantification to infectivity at 28 °C and 37 °C in both cell types.
Tiago Souza Salles, Erica Santos Martins-Duarte, Marcelo Damião Ferreira de Meneses, Monica Ferreira Moreira, Davis Fernandes Ferreira, Renata Campos Azevedo, Wanderley De Souza, Lucio Ayres Caldas   +7 more
openaire   +3 more sources

The transient expression of CHIKV VLP in large stirred tank bioreactors

Cytotechnology, 2019
Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918-940, 2018).
Peifeng Chen, Jacob Demirji, Vera B. Ivleva, Joe Horwitz, Richard Schwartz, Frank Arnold   +5 more
openaire   +3 more sources

Summary of the CHIKV ligands that were detected by MS/MS analysis in the rVACV-CHIKV infected cells.

, 2019
Summary of the CHIKV ligands that were detected by MS/MS analysis in the rVACV-CHIKV infected cells.
Elena Lorente (301126), Mariano Esteban (50256), Concepción Palomo (760052), Arie Admon (394782), Carmen Mir (479004), Juan García-Arriaza (207920), Daniel López (279575), Eilon Barnea (394777), Alejandro Barriga (394779)   +8 more
core   +1 more source

The risk and risk factors of chikungunya virus infection and rheumatological sequelae in a cohort of U.S. Military Health System beneficiaries: Implications for the vaccine era.

PLoS Neglected Tropical Diseases
BackgroundUnderstanding the risk of chikungunya virus (CHIKV) infection and rheumatic sequelae across populations, including travelers and the military, is critical. We leveraged healthcare delivery data of over 9 million U.S. Military Health System (MHS)
Simon Pollett, Hsing-Chuan Hsieh, Dan Lu, Melissa Grance, Stephanie Richard, Gosia Nowak, Charlotte Lanteri, David Tribble, Timothy Burgess   +8 more
doaj   +1 more source

Evolution of CHIKV seroprevalence.

, 2017
Evolution of CHIKV seroprevalence.
Françoise Maire (3636400), Pierre Gallian (749967), Olivier Flusin (3636397), Xavier de Lamballerie (95840), Jacques Chiaroni (473993), Remi Charrel (2280064), Rachid Djoudi (3636403), Isabelle Leparc-Goffart (213294), Pascale Richard (290020), Pierre Tiberghien (175919)   +9 more
core   +1 more source

CHIKV_mos infectivity can be enhanced after replication in mammalian cells.

, 2015
The viral stocks of CHIKVvero-NIH3T3 and CHIKVmos-NIH3T3 were prepared after CHIKVvero or CHIKVmos (Ross strain, MOI = 1) replicated in NIH3T3 for 48 h.
Dhiraj Acharya (815914), Fengwei Bai (139564), John F. Anderson (73440), Faqing Huang (7517), Amber M. Paul (815915)   +4 more
core   +1 more source

CHIKV_mos has reduced infectivity than CHIKV_vero.

, 2015
(A-H) Indicated cells were infected with CHIKVvero or CHIKVmos (Ross strain, MOI = 1) for 24 h to measure gene expression of CHIKV E1 and cellular β-actin by RT-qPCR.
Dhiraj Acharya (815914), Fengwei Bai (139564), John F. Anderson (73440), Faqing Huang (7517), Amber M. Paul (815915)   +4 more
core   +1 more source

IFNs Inhibit CHIKV Infection

, 2013
HeLa cells were pretreated 6 h before infection with the indicated doses (IU/mL) of IFN. IFNα1b , IFNβ1a, and IFNγ were tested. Cells were then exposed to CHIKV at two mois (10 and 1). (A) Inhibition of CHIKV replication. At 24 h pi, HeLa cells
Céline Trouillet (31905), Karin Le Roux (31909), Alain Michault (31924), Marie-Pascale Frenkiel (31912), Philippe V Afonso (31914), Hafida Fsihi (31911), Ali Saïb (31921), Nicoletta Casartelli (31904), Simona Ozden (31916), Nathalie Sol-Foulon (31908), Matthew L Albert (31925), Florence Guivel-Benhassine (31906), Marion Sourisseau (31902), Clémentine Schilte (31903), Alessia Zamborlini (31920), Isabelle Schuffenecker (31918), Dominika Rudnicka (31907), Marie-Christine Prevost (31910), Felix A Rey (31922), Fabien Blanchet (31913), Olivier Schwartz (15284), Bruno Verhasselt (31919), Antoine Gessain (31917), Fernando Arenzana-Seisdedos (18907), Pierre-Emmanuel Ceccaldi (31915), Philippe Desprès (31923)   +25 more
core   +1 more source

Expression of CHIKV glycoproteins.

, 2014
A. Structural proteins in the pCHIKV37997 construct. B. 105 293T cells were transfected with pCHIKV37997 or plasmids expressing CHIKV E1 or EIE2. Total protein was harvested at 2 days post-transfection and subjected to western blotting analysis using the
Dakang Hu (647924), Jin Zhang (53297), Lianhua Yu (647926), Shuangchun Liu (647925), Hua Wang (13530), Ying Qu (437588), Lingfen Sun (647927)   +6 more
core   +1 more source

Live-attenuated CHIKV vaccine with rearranged genome replicates in vitro and induces immune response in mice.

PLoS Neglected Tropical Diseases
Chikungunya fever virus (CHIKV) is a mosquito-borne alphavirus that causes wide-spread human infections and epidemics in Asia, Africa and recently, in the Americas. CHIKV is considered a priority pathogen by CEPI and WHO.
Irina Tretyakova, Joongho Joh, Mary Gearon, Jennifer Kraenzle, Sidney Goedeker, Ava Pignataro, Brian Alejandro, Igor S Lukashevich, Donghoon Chung, Peter Pushko   +9 more
doaj   +1 more source