Results 31 to 40 of about 3,390,395 (296)

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

First-in-human clinical study of an embryonic stem cell product for urea cycle disorders

Stem Cell Research & Therapy
Background This study assesses the safety and efficacy of hepatocyte-like cell (HLC) infusion therapy derived from human embryonic stem cells as bridging therapy for neonatal-onset urea cycle disorders (UCD).
Akihiro Umezawa, Akinari Fukuda, Reiko Horikawa, Hajime Uchida, Shin Enosawa, Yoshie Oishi, Naoko Nakamura, Kengo Sasaki, Yusuke Yanagi, Seiichi Shimizu, Toshimasa Nakao, Tasuku Kodama, Seisuke Sakamoto, Itaru Hayakawa, Saeko Akiyama, Noriaki Saku, Shoko Miyata, Kenta Ite, Palaksha Kanive Javaregowda, Masashi Toyoda, Hidenori Nonaka, Kazuaki Nakamura, Yoshikazu Ito, Yasuyuki Fukuhara, Osamu Miyazaki, Shunsuke Nosaka, Kazuhiko Nakabayashi, Chizuko Haga, Takako Yoshioka, Akira Masuda, Takashi Ohkura, Mayu Yamazaki-Inoue, Masakazu Machida, Rie Abutani-Sakamoto, Shoko Miyajima, Hidenori Akutsu, Yoichi Matsubara, Takashi Igarashi, Mureo Kasahara   +38 more
doaj   +1 more source

A multi-modal MRI analysis of brain structure and function in relation to OXT methylation in maltreated children and adolescents

Translational Psychiatry, 2021
Child maltreatment dysregulates the brain’s oxytocinergic system, resulting in dysfunctional attachment patterns. However, how the oxytocinergic system in children who are maltreated (CM) is epigenetically affected remains unknown.
Shota Nishitani, Takashi X. Fujisawa, Daiki Hiraoka, Kai Makita, Shinichiro Takiguchi, Shoko Hamamura, Akiko Yao, Koji Shimada, Alicia K. Smith, Akemi Tomoda   +9 more
doaj   +1 more source

Between-word junctures in early multi-word speech [PDF]

, 2002
Most children aged 1;6 to 2;0 begin to use utterances of two words or more. It is therefore important for child phonologists to consider the development of phonetic and phonological phenomena that characterize connected speech.
Newton, C., Wells, B.
core   +1 more source

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

A Study of Child Developmental Norms for Children from birth to 5 years of age in Chiang Mai

International Journal of Child Development and Mental Health, 2013
This study aims to determine the developmental norms for children from birth to 5 years of age who live in Chiang Mai and to identify an order of assessment items of a child development tool for children from birth to 5 years of age, of the Department of
Samai Sirithongthaworn, Atsawin Narkpongphun, Nonglak Pongtaweeboon, Pinyo Itsarapong, Wasana Kasemsuk, Suleelak Pila, Preechaya Phrommin, Saowalak Langaphin, Usanee Sutthibuta, Kittiporn Thanu   +9 more
doaj  

Exploring Lifestyles and Sensory Processing Patterns of Toddlers in Relation to Sleep Patterns Using Body Movement Analysis

Clocks & Sleep
This study explored the impact of lifestyle habits and sensory processing patterns on sleep quality by analyzing body movements (BMs) during the first and last 3 h of sleep in toddlers. We collected cross-sectional data about sleep-related habits from 58
Azusa Ono, Yoshiko Iwatani, Arika Yoshizaki, Tomoko Nishimura, Ikuko Mohri, Kuriko Kagitani-Shimono, Masako Taniike   +6 more
doaj   +1 more source

Elevated risk of attention deficit hyperactivity disorder (ADHD) in Japanese children with higher genetic susceptibility to ADHD with a birth weight under 2000 g

BMC Medicine, 2021
Background Both genetic and pre- and perinatal factors, including birth weight, have been implicated in the onset of attention deficit hyperactivity disorder (ADHD) traits among children.
Md Shafiur Rahman, Nagahide Takahashi, Toshiki Iwabuchi, Tomoko Nishimura, Taeko Harada, Akemi Okumura, Nori Takei, Yoko Nomura, Kenji J. Tsuchiya   +8 more
doaj   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

The Study of Child Development Norms of Thai Children Age from Birth to 5 Years using Child Development Assessment Tools Developed by the Mental Health Department, Ministry of Public Health, Thailand

International Journal of Child Development and Mental Health, 2013
This study aims to determine the development norms of Thai children age from birth to 5 years and to compare the assessment items of a child development assessment tool for children age from birth to 5 years, developed by the Department of Mental Health,
Samai Sirithongthaworn, Atsawin Narkpongphun, Nonglak Pongtaweeboon, Pinyo Itsarapong, Wasana Kasemsuk, Suleelak Pila, Preechaya Phrommin, Saowalak Langaphin, Usanee Sutthibuta, Kittiporn Thanu   +9 more
doaj