Results 211 to 220 of about 191,343 (255)

Single‐Injection Multi‐Omics Analysis by Direct Infusion Mass Spectrometry

Angewandte Chemie, EarlyView.
A high‐throughput direct infusion mass spectrometry platform, enabled by gas‐phase ion mobility separation, supports single‐injection analysis of peptides, polar metabolites, and lipids. Coupled with custom software, it identified ∽1,300 proteins and ∽600 metabolites in ∽4.3 minutes per sample, and demonstrated broad utility in macrophage polarization ...
Yuming Jiang, Ivan Salladay‐Perez, Amanda Momenzadeh, Jesús Muñoz‐Estrada, Uktarsh Tripathi, Anthony J. Covarrubias, Jesse G. Meyer   +6 more
wiley   +2 more sources

A platform to design and optimise fluorogenic scFvs for detection of interleukin 33. [PDF]

Chem Sci
Reese AE, Karmakar U, Restori M, Hermoso MA, Church GM, Kuru E, Vendrell M.   +6 more
europepmc   +1 more source

Enhanced Sensitivity of a Modified Quaking‐Induced Conversion Diagnostic Test for the Broad Detection of Sporadic and Inherited Prion Diseases: A Retrospective Study

Annals of Neurology, EarlyView.
Objective Quaking‐induced conversion (QuIC) tests, which detect prion‐seeding activity in cerebrospinal fluid (CSF), have markedly advanced the antemortem diagnosis of prion diseases such as Creutzfeldt‐Jakob disease (CJD). These tests provide high diagnostic accuracy and enable timely differentiation from other rapidly progressive neurodegenerative ...
Jennifer Myskiw, Rebecca Fox, Dominic M.S. Kielich, Lise Lamoureux, Melanie Leonhardt, Olivia Nykvist, Jessy A. Slota, Kristen Avery, Clark Phillipson, Kathy Frost, Sharon Simon, Brian S. Appleby, Ben A. Bailey‐Elkin, Stephanie A. Booth   +13 more
wiley   +1 more source

Multiplexed Tandem Mass Spectrometry Imaging Enables Large‐Scale Isomer Mapping and Annotation in Tissues

Angewandte Chemie, EarlyView.
Parallel mass spectrometry imaging acquires high mass resolution and tandem mass spectrometry images from 108 isolation windows in a single run. Spatial similarity networking clusters product ions by spatial distribution, enabling annotation of isomers and isobars as demonstrated for phospholipids and oxidized cholesterol in mouse and human brain ...
Varun V. Sharma, Gabor Toth, Robert Martinis, Cathrin E. Hansen, Gijs Kooij, Ingela Lanekoff   +5 more
wiley   +2 more sources

Correction to "DNA-Programmable Protein Degradation: Dynamic Control of Proteolysis-Targeting Chimera Activity via DNA Hybridization and Strand Displacement". [PDF]

JACS Au
Kashyap D, Haider S, Milne TA, Booth MJ.
europepmc   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Mechanosensitive channel engineering: A study on the mixing and matching of YnaI and MscS sensor paddles and pores. [PDF]

Nat Commun
Flegler VJ, Rasmussen A, Hedrich R, Rasmussen T, Böttcher B.   +4 more
europepmc   +1 more source

Changing cognitive chimera states in human brain networks with age: Variations in cognitive integration and segregation. [PDF]

PLoS Comput Biol
Patton A, Davidsen J.
europepmc   +1 more source

A Semi-Automated and Unbiased Microglia Morphology Analysis Following Mild Traumatic Brain Injury in Rats. [PDF]

Int J Mol Sci
Sumberg L, Berman R, Pazgier A, Torres J, Qiu J, Tran B, Greene S, Atwood R, Boese M, Choi K.   +9 more
europepmc   +1 more source

A versatile platform for combinatorial antibody library cloning and NGS-based quality control with high accuracy. [PDF]

Nucleic Acids Res
Zhang Z, D'Hondt S, Van der Kant R, Hermans G, Geukens N, Pinheiro VB, Schymkowitz J, Rousseau F, Dewilde M.   +8 more
europepmc   +1 more source