Results 51 to 60 of about 103,657 (215)

Tuning Mechanoreceptor Signaling Enhances the Adjuvant Effect of Biomaterial Vaccines

Advanced Functional Materials, EarlyView.
Matrix stiffness serves as an independent immunological signal in biomaterial vaccines. Stiffer microporous hydrogel vaccines enhance dendritic cell maturation and migration to draining lymph nodes, promote T follicular helper cell expansion through Yap/Taz signaling, and increase antigen‐specific antibody production.
Lily Shang, Daniel P. Mayer, Prabhat K. Purbey, Yingzi Liu, Kindra Kelly‐Scumpia, Clara Kang, Natalie Tsubamoto, Dhriti Bolangadi, Iqra Arshad, Charisse Ng, Maksim V. Plikus, Dino Di Carlo, Jason S. Weinstein, Philip O. Scumpia   +13 more
wiley   +1 more source

7C: Computational Chromosome Conformation Capture by Correlation of ChIP-seq at CTCF motifs

BMC Genomics, 2019
Background Knowledge of the three-dimensional structure of the genome is necessary to understand how gene expression is regulated. Recent experimental techniques such as Hi-C or ChIA-PET measure long-range chromatin interactions genome-wide but are ...
Jonas Ibn-Salem, Miguel A. Andrade-Navarro   +1 more
doaj   +1 more source

Decellularized Extracellular Matrix (dECM) in Tendon Regeneration: A Comprehensive Review

Advanced Healthcare Materials, EarlyView.
Decellularized Extracellular Matrix (dECM) offers a promising solution by replicating the native tendon microenvironment and promoting regeneration. This review highlights advances in the decellularization methods, as well as their integration with emerging technologies and translational progress in tendon tissue engineering.
Kumaresan Sakthiabirami, Yeonwoo Park, Seong Kyeong Jo, Min Hee Kim, Jae Hwang Song   +4 more
wiley   +1 more source

aFARP-ChIP-seq, a convenient and reliable method for genome profiling in as few as 100 cells with a capability for multiplexing ChIP-seq

Epigenetics, 2019
Much effort has been devoted to understand how chromatin modification regulates development and disease. Despite recent progress, however, it remains difficult to obtain high-quality epigenomic maps using chromatin-immunoprecipitation-coupled deep ...
Wenbin Liu, Sibiao Yue, Xiaobin Zheng, Minjie Hu, Jia Cao, Yixian Zheng   +5 more
doaj   +1 more source

RACS: rapid analysis of ChIP-Seq data for contig based genomes

BMC Bioinformatics, 2019
Background Chromatin immunoprecipitation coupled to next generation sequencing (ChIP-Seq) is a widely-used molecular method to investigate the function of chromatin-related proteins by identifying their associated DNA sequences on a genomic scale.
Alejandro Saettone, Marcelo Ponce, Syed Nabeel-Shah, Jeffrey Fillingham   +3 more
doaj   +1 more source

Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential

Advanced Healthcare Materials, EarlyView.
This review examines how cellular behavior is regulated by mechanical cues transmitted through soft biomaterials, from single‐cell mechanosensing to tissue‐level adaptation. It highlights why physiological relevance, rather than model complexity alone, is critical for translational mechanobiology and introduces a scoring framework linking material ...
Mathias Polz, Manuel P. Kainz, Niklas Haider, Theresa Rienmüller, Kerstin Lenk, Rupert Kargl, Michele Terzano, Gerhard Sommer, Gerhard A. Holzapfel, Julia Fuchs   +9 more
wiley   +1 more source

ChIP-chip versus ChIP-seq: Lessons for experimental design and data analysis

BMC Genomics, 2011
Background Chromatin immunoprecipitation (ChIP) followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq) allows genome-wide discovery of protein-DNA interactions such as transcription factor bindings and histone ...
Karchenko Peter V, Bishop Eric, Ho Joshua WK, Nègre Nicolas, White Kevin P, Park Peter J   +5 more
doaj   +1 more source

Reprogramming the Immunosuppressive Microenvironment in Triple‐Negative Breast Cancer via FAP‐Targeted Nanoprobes for Multimodal Imaging and Photothermal Therapy

Advanced Materials, EarlyView.
Targeting cancer‐associated fibroblasts (CAFs) with fibroblast activation protein (FAP)‐directed nanoprobes for multimodal imaging and photothermal remodeling of the immunosuppressive microenvironment to enhance immunotherapy in triple‐negative breast cancer (TNBC).
Ling Zhan, Yanhong Chen, Jingjing Hu, Chunting Wang, Huanhuan Liu, Defan Yao, Dengbin Wang   +6 more
wiley   +1 more source

ATAC2GRN: optimized ATAC-seq and DNase1-seq pipelines for rapid and accurate genome regulatory network inference

BMC Genomics, 2018
Background Chromatin accessibility profiling assays such as ATAC-seq and DNase1-seq offer the opportunity to rapidly characterize the regulatory state of the genome at a single nucleotide resolution.
Thomas J. F. Pranzatelli, Drew G. Michael, John A. Chiorini   +2 more
doaj   +1 more source

Stromal‐Derived IL‐8 Promotes M2 Macrophage Polarization via the RhoA/MRTF‐A/SRF Transcriptional Axis to Impair CD8+ T Cell Cytotoxicity in Lung Cancer

Advanced Science, EarlyView.
In the recondite pulmonary neoplastic milieu, DESMIN+ cancer‐associated fibroblasts function as the paramount stromal source of IL‐8, precipitating a deleterious immunological metamorphosis by engaging CXCR2 receptors on the myeloid lineage. This paracrine stimulus triggers a mechanotransduction cascade characterized by RhoA‐dependent actin ...
Xuyu Gu, Qiyu Fang, Jia Yu, Ting Yu, Huashan Shi, Kaiqi Jin, Lei Jiang, Wentian Zhang   +7 more
wiley   +1 more source