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Detection of Chlamydiaceae DNA in veterinary specimens using a family-specific PCR

Letters in Applied Microbiology, 2007
The aim of this work was to develop a rapid molecular test for the detection of the Chlamydiaceae family, irrespective of the species or animal host.The method described herein is a polymerase chain reaction targeting the 16S rRNA gene of the Chlamydiaceae family, and the results demonstrate that the test reacts with five reference Chlamydiaceae but ...
Condon, K., Oakey, J.
exaly   +5 more sources

A new amplification target for PCR?RFLP detection and identification of Chlamydiaceae species

Archives of Microbiology, 2005
The family Chlamydiaceae contains nine species pathogenic to humans and animals, but their routine identification is hampered by inadequate detection methods. In an attempt to find a new region for PCR detection and discrimination of the Chlamydiaceae species, the 3' end of the omp2 gene of Chlamydiaceae has been examined.
Vladimir V Demkin
exaly   +4 more sources

High-yield culture and purification of Chlamydiaceae bacteria

Journal of Microbiological Methods, 2005
Research on intracellular bacteria of the family Chlamydiaceae, and the diseases they cause, requires large amounts of infectious elementary bodies (EB). We describe an approach that maximizes the generation of Chlamydia pneumoniae, Chlamydia trachomatis, Chlamydia abortus, or Chlamydia pecorum EBs in several replication cycles over approximately 10 ...
Teayoun Kim   +5 more
openaire   +2 more sources

Inclusion proteins of Chlamydiaceae.

Drugs of today (Barcelona, Spain : 1998), 2006
Chlamydiaceae are obligate intracellular pathogens with family members among the etiological agents of several human diseases, such as blinding trachoma, sexually transmitted disease (Chlamydia trachomatis) and pneumonia (Chlamydophila pneumoniae, Chlamydophila psittaci).
Y, Pannekoek, A, van der Ende
openaire   +1 more source

A tale of discrimination: Differentiation of chlamydiaceae by polymerase chain reaction

Seminars in Avian and Exotic Pet Medicine, 2000
We developed a nested, multiplex polymerase chain reaction (PCR) for simultaneous detection and discrimination of three species of chlamydia in human and avian specimens to determine transmission of chlamydophila psittaci from infected birds to humans.
Trudy O. Messmer   +3 more
openaire   +2 more sources

Occurrence of Chlamydiaceae in non-symptomatic free-living raptors in Spain

European Journal of Wildlife Research, 2011
Few studies have investigated the role of raptors as natural reservoirs of Chlamydiaceae spp. and the preferred anatomical sites where these bacteria can be detected in non-symptomatic wild birds. We investigated the occurrence of Chlamydiaceae in 54 non-symptomatic adult free-living birds belonging to 14 species sampled upon reception in a raptor ...
N. Ortega   +4 more
openaire   +2 more sources

Immunoblotting, ELISA and culture evidence for Chlamydiaceae in sows on 258 Belgian farms

Veterinary Microbiology, 2004
The prevalence of Chlamydiaceae infections on 258 closed pig breeding farms in Belgium was examined. For this purpose, 258 farms were randomly selected in the provinces West-Vlaanderen (44%), Oost-Vlaanderen (20%), Antwerpen (10%) and Vlaams-Brabant (6%).
Ann Desplanques   +8 more
openaire   +3 more sources

Molecular evidence for genetic distinctions between Chlamydiaceae detected in Siamese crocodiles (Crocodylus siamensis) and known Chlamydiaceae species.

The Japanese journal of veterinary research, 2015
Chlamydiosis, caused by Chlamydiaceae, is a zoonotic disease found in humans and several species of animals, including reptiles and amphibians. Although chlamydiosis in saltwater crocodiles has been previously reported in South Africa and Papua New Guinea, the reported strains have not been identified or confirmed. Therefore, the main aim of this study
Sariya, Ladawan   +6 more
openaire   +2 more sources

Detection of a new Chlamydiaceae strain from turkeys in Croatia

2012
Obligate intracellular bacterium Chlamydia psittaci (C. psittaci) is an important etiological agent in turkey respiratory diseases. It is commonly found in Europe, causing economical losses and present a possible threat for human health. Health problems occurred on the fattening turkey’s farm, particularly related to the respiratory system over several
Prukner-Radovčić, Estella   +2 more
openaire   +3 more sources

Emendation of the family Chlamydiaceae: proposal of a single genus, Chlamydia, to include all currently recognized species.

Systematic and Applied Microbiology, 2015
K. Sachse   +6 more
semanticscholar   +1 more source

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