Results 151 to 160 of about 759,473 (295)

A TAF10‐ERF109 Transcriptional Module Directs Flavonoid‐Based Stress Resilience and Yield Enhancement in Foxtail Millet and Wheat

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT To ensure sustainable agricultural production under escalating environmental constraints such as drought and salinity, innovative strategies are urgently needed. Here, we reveal in foxtail millet (Setaria italica) that transcription factors TAF10 and ERF109 form a functional complex which co‐activates the expression of key flavonoid ...
Meng Zhang   +15 more
wiley   +1 more source

Targeted Modulation of Eukaryotic Release Factor 1 (OseRF1) by an RNA Aptamer Enhances Drought Adaptation in Rice

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Drought stress severely restricts rice productivity, necessitating strategies to enhance stress resilience. Here, we identify translation termination as a tunable regulatory node for stress adaptation. Using 25 rounds of SELEX, we isolated RTAR, an RNA aptamer that binds the rice eukaryotic release factor 1 (OseRF1) with nanomolar affinity ...
Shuangfeng Dai   +7 more
wiley   +1 more source

RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee   +3 more
wiley   +1 more source

Evolution‐Inspired Engineering of Diterpene Biosynthesis via Chloroplast Genome Modification

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Terpenes constitute the largest and most structurally diverse class of plant secondary metabolites, with critical roles in plant‐environment interactions and broad industrial applications. Although nuclear genome engineering of terpene pathways has been extensively explored, chloroplast genome engineering remains largely undeveloped, with most
Alessandro Occhialini   +6 more
wiley   +1 more source

Converging GWAS and Genome Editing Decrypts MsCBF8‐MsGolS2 Regulatory Module for Drought Tolerance Without Yield Penalty in Medicago sativa

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Drought constitutes a major abiotic stress limiting alfalfa productivity. To elucidate the genetic basis of drought tolerance, a genome‐wide association study (GWAS) was conducted in alfalfa, which identified a CBF/DREB1 family transcription factor designated MsCBF8.
Yangyang Zhang   +11 more
wiley   +1 more source

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