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The Structure of Chloroplast DNA
Annual Review of Plant Physiology, 1979INTRODUCTION . PHYSICAL STUDIES ON THE STRUCTURE OF ctDNA . Kinetic Comple;city of ctDNA . Circularity of ctDNA .
J R Bedbrook, R Kolodner
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Journal of Experimental Botany, 1989
The influence of two DNA gyrase inhibitors, nalidixic acid and novobiocin on DNA synthesis in isolated pea chloroplasts was examined by autoradiography and ...
W. RONALD MILLS +3 more
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The influence of two DNA gyrase inhibitors, nalidixic acid and novobiocin on DNA synthesis in isolated pea chloroplasts was examined by autoradiography and ...
W. RONALD MILLS +3 more
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Isolation of Maize Chloroplasts and Chloroplast DNA
1994This procedure is for the isolation of chloroplasts and highly purified chloroplast DNA from 1 kg of maize seedling leaves. Because starch reduces the yield of intact chloroplasts, light-grown seedlings are placed into darkness for 1–2 days prior to DNA isolation. All manipulations should be performed at 4°C, unless otherwise noted.
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Chloroplast ribosomal RNA genes in the chloroplast DNA of Euglena gracilis
Journal of Molecular Biology, 1973Euglena chloroplast DNA has a buoyant density in CsCI of 1.686. Shearing this DNA produces a satellite band at density 1.700. The satellite, easily lost during preparative CsCI gradient centrifugation of chloroplast DNA, contains the genes for chloroplast ribosomal RNA. Pure Euglena chloroplast DNA is shown to contain one set of ribosomal RNA genes for
J R, Rawson, R, Haselkorn
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The characteristics of spinach chloroplast DNA polymerase
Archives of Biochemistry and Biophysics, 1969Purified chloroplast preparations from spinach incorporate [3H]-dTTP1 into an acid-insoluble product. The incorporation reaction is highly dependent on all four deoxynucleoside triphosphates, Mg2+ and a sulphydryl reagent, and is further stimulated by KCl.
D, Spencer, P R, Whitfeld
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Chloroplast DNA from three archegoniates
Planta, 19801. DNA from female and male Sphaerocarpos donnellii (liverwort) plants exhibits at least two species with buoyant densities of 1.703 (main band) and 1.691 (satellite) g cm(-3) in CsCl equilibrium gradients. At least part, if not all, of the satellite DNA is localized in plastids.
R G, Herrmann, H K, Palta, K V, Kowallik
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The kinetic complexity of Acetabularia chloroplast DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978The kinetic complexity of Acetabularia cliftonii chloroplast DNA is 1.52 +/- 0.26 . 10(9) daltons, compared to 0.2 .10(9) daltons for Chlamydomonas chloroplast DNA. There is an average of three genomes per chloroplast. The unusually large size of the Acetabularia genome may reflect the ancient evolutionary history of this organism.
U, Padmanabhan, B R, Green
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1977
We had previously reported the isolation of circular chloro-plast (et) DNA molecules from pea leaves. Circular pea ctDNA was found to have a molecular weight of 90×106 with no evidence of inter- or intramolecular heterogeneity. Recently we have extensively studied the size and structure of ctDNAs from pea, bean, spinach, lettuce, corn, and oats.2 As ...
K. K. Tewari +3 more
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We had previously reported the isolation of circular chloro-plast (et) DNA molecules from pea leaves. Circular pea ctDNA was found to have a molecular weight of 90×106 with no evidence of inter- or intramolecular heterogeneity. Recently we have extensively studied the size and structure of ctDNAs from pea, bean, spinach, lettuce, corn, and oats.2 As ...
K. K. Tewari +3 more
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Chloroplast DNA and cytoplasmic male-sterility
Theoretical and Applied Genetics, 1983This paper deals with the relationship of chloroplast DNA (ctDNA) and cytoplasmic malesterility (CMS) of maize, wheat and rape. Intramolecular heterogeneity and fragment patterns of DNA digested with restriction endonucleases were compared and analyzed. For this purpose methods of agarose gel electrophoresis and two dimensional gel electrophoresis with
L, Jigeng, L, Yi-Nong
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Trends in Biochemical Sciences, 1978
Twenty years after the discovery that chloroplasts contain DNA we still do not know the significance of approximately 90% of this DNA.
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Twenty years after the discovery that chloroplasts contain DNA we still do not know the significance of approximately 90% of this DNA.
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