Electroformation of Giant Unilamellar Vesicles from Damp Films in Conditions Involving High Cholesterol Contents, Charged Lipids, and Saline Solutions [PDF]
MembranesGiant unilamellar vesicles (GUVs) are frequently used as membrane models in studies of membrane properties. They are most often produced using the electroformation method.
Ivan Mardešić +2 more
doaj +2 more sources
Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content [PDF]
MembranesGiant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Ivan Mardešić +2 more
doaj +2 more sources
Protein‐Based Patterning to Spatially Functionalize Biomimetic Membranes
Small Methods, Volume 7, Issue 12, December 15, 2023., 2023 In this report, the authors test the capabilities of the MinDE protein system to organize molecules of different natures and complexities into distinct patterns. Techniques spanning from two‐photon direct laser writing, lipid membrane coating of surfaces, and protein encapsulation in lipid vesicles are employed to demonstrate that this protein‐based ...
María Reverte‐López +6 more
wiley +1 more source
Small Methods, Volume 7, Issue 12, December 15, 2023., 2023 Giant unilamellar vesicles (GUVs) have emerged as suitable containers for the bottom‐up construction of artificial cells, with numerous methods available for their production. This review discusses the state of the art in different GUV production methods and their compatibility with artificial cell requirements, as well as operational requirements such
Lori Van de Cauter +3 more
wiley +1 more source
Martini 3 Coarse‐Grained Force Field: Small Molecules
Advanced Theory and Simulations, Volume 5, Issue 1, January 2022., 2022 We describe how small molecules can be accurately parametrized within the recently developed Martini 3 coarse‐grained force field and present a database of validated small molecule models. The database, along with the outlined model design strategies and the modularity of the Martini force field, pave the way for the investigation of complex ...
Riccardo Alessandri +6 more
wiley +1 more source
FEBS Letters, Volume 595, Issue 16, Page 2127-2146, August 2021., 2021 To disentangle the elusive lipid–protein interactions in T‐cell activation, we investigate how externally imposed variations in mobility of key membrane proteins (T‐cell receptor [TCR], kinase Lck, and phosphatase CD45) affect the local lipid order and protein colocalisation.
Iztok Urbančič +10 more
wiley +1 more source
Membrane Engineering: Phase Separation in Polymeric Giant Vesicles
Small, Volume 16, Issue 27, July 9, 2020., 2020 One characteristic of cell membrane is an elaborate lipid patterning to carry out essential cellular functions. While lipid–lipid separation within giant unilammelar vesicles (GUVs) is studied, polymer–polymer hybrid GUVs remain evasive. Here, the thermodynamically driven phase separation of amphiphilic polymers from film topology to microscale self ...
Emeline Rideau +2 more
wiley +1 more source
Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion. [PDF]
Membranes (Basel), 2023 Boban Z +5 more
europepmc +1 more source
Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains. [PDF]
Membranes (Basel), 2023 Mardešić I +3 more
europepmc +1 more source
Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results. [PDF]
Membranes (Basel), 2021 Boban Z +3 more
europepmc +1 more source