Results 31 to 40 of about 740,585 (289)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

open access: yesFEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Metal-Organic Frameworks Characterization via Inverse Pulse Gas Chromatography

open access: yesApplied Sciences, 2021
The desire to customize the properties of a material through complete control over both its chemical and architectural structure has created a constant and persistent need for efficient and convenient characterization techniques.
Kareem Yusuf   +3 more
doaj   +1 more source

Electrochemical Process for Diazinon Removal from Aqueous Media: Design of Experiments, Optimization, and DLLME-GC-FID Method for Diazinon Determination [PDF]

open access: yes, 2015
In the present study, electrochemical process was studied via removal of diazinon (O,O-diethyl O-2-isopropyl-6-methylpyrimidin-4-yl phosphorothioate) as an insecticide/ acaricide organic case study.
Daraei, H.   +4 more
core   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

open access: yesFEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan   +6 more
wiley   +1 more source

Hot-wire detector for chemically active materials used in gas chromatography [PDF]

open access: yes, 1966
Hot-filament detector analyzes chemically active materials used in gas chromatography. The detector reacts chemically with the effluent vapors in the gas chromatographic apparatus to change the electrical resistance of the filament as a function of the ...

core   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

open access: yesFEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso   +4 more
wiley   +1 more source

Wave Mathematical Model to Describe Gas Chromatography

open access: yesHolodilʹnaâ Tehnika i Tehnologiâ, 2017
This paper describes the mathematical model of concentration waves passing through a layer of adsorbent. The analytic solution to this model deduced for eigenwaves of adsorptive layer had been found.
M. B. Kravchenko
doaj   +1 more source

Fuels characterization studies [PDF]

open access: yes, 1980
Current analytical techniques used in the characterization of broadened properties fuels are briefly described. Included are liquid chromatography, gas chromatography, and nuclear magnetic resonance spectroscopy.
Antoine, A. C.   +2 more
core   +1 more source

Toxicological Analysis of Some Drugs of Abuse in Biological Samples [PDF]

open access: yes, 2015
Consumption of drugs of abuse is a scourge of modern world. Abuse, drug addiction and their consequences are one of the major current problems of European society because of the significant repercussions in individual, family, social and economic level ...
Baconi, Daniela Luiza   +5 more
core   +3 more sources

Home - About - Disclaimer - Privacy