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Determination of Thiopental by High Pressure Liquid Chromatography
Acta Pharmacologica et Toxicologica, 1979AbstractA specific and rapid reverse phase HPLC‐method for the determination of thiopental in serum and in protein free solutions is described. No extraction is used. The detection limit is about 0.090 μg/ml for serum and about 0.030 μg/ml for buffer solutions. The retention time is 3.80 min.
J. Heslop Christensen +1 more
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Forensic aspects of high-pressure liquid chromatography
Journal of Chromatography A, 1976This paper reviews the applications of high-pressure liquid chromatography (HPLC) to forensic problems, and discusses some of the developments that have taken place in the use of the technique in the Metropolitan Police Laboratory. Preparation of octadecyltrichlorosilane-modified silica is described and some of the chromatographic characteristics of ...
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A fluorescence detector for high-pressure liquid chromatography
Journal of Chromatography A, 1977We describe a fluorescence spectrophotometer adapted with a micro quartz flow cell to record the output of modern liquid chromatographs. The optical system is double beam in that the light source variations are cancelled out by a second photomultiplier, thus enhancing the sensitivity of the technique. The emission spectra may be scanned by stopping the
W. Slavin +2 more
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Separation of cytokinins by High Pressure Liquid Chromatography
Planta, 1975A number of cytokinin reference compounds have been successfully separated by High Pressure Liquid Chromatography using columns of pellicular strong cation exchange resin and of pellicular polyamide. On polyamide, all cytokinins were eluted within 10 min with an aqueous buffer but on the cation exchange resin some cytokinins (generally those with bulky
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High-back-pressure liquid chromatography
Journal of Chromatography A, 1982Abstract The retention behaviour of solutes on various stationary phases was studied by micro-high-performance liquid chromatography using liquefied solvents as the mobile phase. An octadecylsilylated stationary phase discriminates between coplanar and non-planar aromatic hydrocarbons in the normal-phase system using isobutane as the mobile phase ...
Daido Ishii, Toyohide Takeuchi
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Journal of Chromatography A, 2014
This is the first investigation in a series that aims to enhance the scientific knowledge needed for reliable analytical method transfer between HPLC and UHPLC using the quality by design (QbD) framework. Here, we investigated the differences and similarities from a thermodynamic point of view between RP-LC separations conducted with 3.5μm (HPLC) and 1.
Krzysztof Kaczmarski +4 more
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This is the first investigation in a series that aims to enhance the scientific knowledge needed for reliable analytical method transfer between HPLC and UHPLC using the quality by design (QbD) framework. Here, we investigated the differences and similarities from a thermodynamic point of view between RP-LC separations conducted with 3.5μm (HPLC) and 1.
Krzysztof Kaczmarski +4 more
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Pressurized solvent reservoir for high-pressure liquid chromatography [PDF]
Journal of Chemical Education, 1982 A pressurized solvent reservoir for high-pressure liquid chromatography that slightly pressurizes the mobile phase before entry into the pumping system and also filters the liquid at the same time.
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High Pressure Liquid Chromatography of Eicosanoids
1985Arachidonic acid (20:4) and other polyunsaturated fatty acids can be oxygenated in many tissues by cyclooxygenase or a variety of lipoxygenases. The intermediates formed in these reactions are then converted enzymatically to a variety of other products.
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High-pressure liquid chromatography of androgens
Journal of Chromatography A, 1979Erich Heftmann +2 more
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ChemInform Abstract: LIQUID‐LIQUID PARTITION COEFFICIENTS BY HIGH‐PRESSURE LIQUID CHROMATOGRAPHY
Chemischer Informationsdienst, 1975Lipid-water partition values can be rapidly and reliably measured by high-pressure liquid chromatography (HPLC) on bonded octadecylsialne supports. This method has been developed and applied to a family of hypotensive triaminopyrimidine 3-oxides. The measured column retention values for this class of compounds correlate well with the values calculated ...
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