Results 311 to 320 of about 1,583,880 (361)
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Determination of hydroxyproline by high pressure liquid chromatography
Analytical Biochemistry, 1992A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated from other amino acids and detected by absorption at 254
Kathleen Reagan, Graham D. Green
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Journal of Separation Science, 2012
Effects of extra-column volume on apparent separation parameters were studied in ultra-high pressure liquid chromatography with columns and inlet connection tubings of various internal diameters (id) using 50-mm long columns packed with 1.8-μm particles ...
N. Wu +3 more
semanticscholar +1 more source
Effects of extra-column volume on apparent separation parameters were studied in ultra-high pressure liquid chromatography with columns and inlet connection tubings of various internal diameters (id) using 50-mm long columns packed with 1.8-μm particles ...
N. Wu +3 more
semanticscholar +1 more source
Carcinogenesis, 1980
Methods were developed for the efficient routine degradation and fractionation of ethylated and methylated DNA. Alkylated DNA was hydrolyzed by a neutral thermal method to yield 3- and 7- alkylpurines and O2-alkylcytosines. The partially apurinic DNA was
D. Beranek +2 more
semanticscholar +1 more source
Methods were developed for the efficient routine degradation and fractionation of ethylated and methylated DNA. Alkylated DNA was hydrolyzed by a neutral thermal method to yield 3- and 7- alkylpurines and O2-alkylcytosines. The partially apurinic DNA was
D. Beranek +2 more
semanticscholar +1 more source
Anthracycline Assay by High-pressure Liquid Chromatography
Journal of Pharmaceutical Sciences, 1981A general method of analysis of anthracycline concentrations was developed. Drug is extracted from plasma with organic solvent and separated from metabolites by high-pressure liquid chromatography on an aminocyanosilica column. Detection and quantitation are by the endogenous fluorescence of compounds having an intact tetracyclic ring structure. Limits
Steven D. Averbuch +4 more
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High pressure liquid chromatography of neutral glycosphingolipids
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972Abstract A system for the separation of neutral glycosphingolipids by high pressure liquid chromatography of their benzoylated derivatives has been devised. Serum mono-, di-, tri-and tetraglycosylceramides are completely resolved within 25 min. A full scale recorder response can be obtained with approximately 1.0 nmole of glycolipid.
James E. Evans, Robert H. McCluer
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Analysis of Trisulfapyrimidines by High-Pressure Liquid Chromatography
Journal of Pharmaceutical Sciences, 1973Abstract: The application of high-pressure liquid chromatography to the separation and analysis of trisulfapyrimidines in pharmaceutical dosage forms is demonstrated. The preparation of samples of both tablet and suspension dosage forms is simple and rapid.
Henry H. Pu, Raymond B. Poet
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Biochemistry, 1978
We have combined the use of a nonprotein carrier (Polybrene), high pressure liquid chromatography, and modifications in Edman chemistry with the improvements of a commercial spinning cup sequenator suggested by Wittmann-Liebold [Wittmann-Liebold, B ...
M. Hunkapiller, L. Hood
semanticscholar +1 more source
We have combined the use of a nonprotein carrier (Polybrene), high pressure liquid chromatography, and modifications in Edman chemistry with the improvements of a commercial spinning cup sequenator suggested by Wittmann-Liebold [Wittmann-Liebold, B ...
M. Hunkapiller, L. Hood
semanticscholar +1 more source
Mexiletine in Plasma by High Pressure Liquid Chromatography
Therapeutic Drug Monitoring, 1981We report here a novel high pressure liquid chromatographic (HPLC) technique for use in therapeutic monitoring of the primary amine antiarrhythmic drug mexiletine in plasma samples. Mexiletine and its structural analogue, the internal standard Ko 768, are extracted with heptane after alkalinization of the sample. The extracts are evaporated to dryness,
Christmore D +4 more
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Assay of Cefotaxime by High-Pressure-Liquid Chromatography
Chemotherapy, 1981A high-pressure-liquid chromatographic (HPLC) procedure for quantitative assay of cefotaxime (CT) and its major metabolite in serum of normal individuals, desacetyl cefotaxime (DACT), is described. It employs Lichrosorb RP-8, elution with phosphoric-acid-methanol and UV absorption at 310 nm.
Tom Bergan, Rita Solberg
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Determination of Thiopental by High Pressure Liquid Chromatography
Acta Pharmacologica et Toxicologica, 1979AbstractA specific and rapid reverse phase HPLC‐method for the determination of thiopental in serum and in protein free solutions is described. No extraction is used. The detection limit is about 0.090 μg/ml for serum and about 0.030 μg/ml for buffer solutions. The retention time is 3.80 min.
J. Heslop Christensen +1 more
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