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Trends in High Pressure Liquid Chromatography

Berichte der Bunsengesellschaft für physikalische Chemie, 1973
AbstractThe recent developments of high pressure liquid chromatography are reviewed with the emphasis on the main problems with which the users of this technic are faced: the design of more sensitive, specific detectors, the design of systems to prepare reproducible solvent gradients and to detect small concentrations of compounds in a variable ...
openaire   +2 more sources

Quantitation of amphotericin B with use of high-pressure liquid chromatography.

Journal of Infectious Diseases, 1977
A chemical method for determination of concentrations of amphotericin B in serum and cerebrospinal fluid (CSF) is described. After extraction with methanol, the antibiotic was separated by reverse-phase, high-pressure liquid chromatography and ...
I. Nilsson-ehle   +4 more
semanticscholar   +1 more source

Yeast mutants blocked in removing the methyl group of lanosterol at C-14. Separation of sterols by high-pressure liquid chromatography.

Biochemistry, 1977
Sterols of a nystatin resistant mutant of the wild type parent of Saccharomyces cerevisiae were separated by a newly developed procedure involving high-pressure liquid chromatography and were identified.
P. Trocha, S. Jasne, D. B. Sprinson
semanticscholar   +1 more source

High-pressure liquid chromatography of steroidal alkaloids

Journal of Chromatography A, 1976
High-pressure liquid chromatography was used to separate the following steroidal alkaloids: tomatidine, solanidine, solasodine, rubijervine, veratramine and jervine. The method was used to prepare crystalline solanidine from a crude mixture of aglycones obtained from Solanum chacoense, and to separate radioactive solanidine from extracts of potato ...
Joseph R. Wagner   +3 more
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Novel method for determination of partition coefficients of penicillins and cephalosporins by high-pressure liquid chromatography.

Journal of Pharmacy and Science, 1977
Newly defined lipophilic indexes, log k', of a series of penicillins and cephalosporins were rapidly and reliably determined by reversed-phase high-pressure liquid chromatography (HPLC) on bonded octadecylsilane supports.
T. Yamana   +3 more
semanticscholar   +1 more source

A fluorescence detector for high-pressure liquid chromatography

Journal of Chromatography A, 1977
We describe a fluorescence spectrophotometer adapted with a micro quartz flow cell to record the output of modern liquid chromatographs. The optical system is double beam in that the light source variations are cancelled out by a second photomultiplier, thus enhancing the sensitivity of the technique. The emission spectra may be scanned by stopping the
W. Slavin   +2 more
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Separation of cytokinins by High Pressure Liquid Chromatography

Planta, 1975
A number of cytokinin reference compounds have been successfully separated by High Pressure Liquid Chromatography using columns of pellicular strong cation exchange resin and of pellicular polyamide. On polyamide, all cytokinins were eluted within 10 min with an aqueous buffer but on the cation exchange resin some cytokinins (generally those with bulky
openaire   +3 more sources

Plasma catecholamines in resting rainbow trout, Salmo gairdneri Richardson, by high pressure liquid chromatography*

, 1982
Plasma norepinephrine and epinephrine from cannulated trout were measured by high pressure liquid chromatography with electrochemical detection. The catecholamines were extracted with acid-washed alumina using a microfilter assembly which permitted ...
J. Woodward
semanticscholar   +1 more source

High-back-pressure liquid chromatography

Journal of Chromatography A, 1982
Abstract The retention behaviour of solutes on various stationary phases was studied by micro-high-performance liquid chromatography using liquefied solvents as the mobile phase. An octadecylsilylated stationary phase discriminates between coplanar and non-planar aromatic hydrocarbons in the normal-phase system using isobutane as the mobile phase ...
Daido Ishii, Toyohide Takeuchi
openaire   +4 more sources

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