Results 41 to 50 of about 3,926,454 (337)

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Journal of Separation Science, 2017
Online comprehensive two‐dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences).
Bob W. J. Pirok, Andrea F. G. Gargano, P. Schoenmakers   +2 more
semanticscholar   +1 more source

Preparation characterization and non-isothermal decomposition kinetics of different carbon nitride sheets

Egyptian Journal of Petroleum, 2020
Three series of graphitic carbon nitride g-C3N4 namely bulk (BCN), sulfur-doped (SCN) and thin layer (TCN) sheets are prepared using urea precursor under a microwave thermal pyrolysis. Several analytical techniques are used to characterize the obtained g-
M. Elshafie, S.A. Younis, P. Serp, E.A.M. Gad   +3 more
doaj   +1 more source

Multidimensional Chromatography and Its Applications in Food Products, Biological Samples and Toxin Products: A Comprehensive Review

Separations, 2022
Food, drugs, dyes, extracts, and minerals are all made up of complex elements, and utilizing unidimensional chromatography to separate them is inefficient and insensitive.
Ruhul Amin, Faruk Alam, Biplab Kumar Dey, Jithendar Reddy Mandhadi, Talha Bin Emran, Mayeen Uddin Khandaker, Sher Zaman Safi   +6 more
doaj   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

BMC Complementary Medicine and Therapies, 2020
Background Ethnobotanical and plant-based products allow for the isolation of active constituents against a number of maladies. Monotheca buxifolia is used by local communities due to its digestive and laxative properties, as well as its ability to cure ...
Joham Sarfraz Ali, Hammad Saleem, Abdul Mannan, Gokhan Zengin, Mohamad Fawzi Mahomoodally, Marcello Locatelli, Syafiq Asnawi Zainal Abidin, Nafees Ahemad, Muhammad Zia   +8 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

A Novel High-Throughput and Sensitive Electrochemiluminescence Immunoassay System

Bioengineering
(1) Background: In vitro diagnostic (IVD) tests are the main means of obtaining diagnostic information for clinical purposes. The electrochemiluminescence immunoassay (ECLIA) has become an important in vitro diagnostic technique. It has unique advantages
Xiancheng Liu, Shuang Zhao, Yan Xu, Bo Zhang, Junli Huang, Feng Liu, Ning Yang, Wenhua Lu, Dandan Shi, Dezhong Xie, Yuanfang Hou, Guixue Wang   +11 more
doaj   +1 more source

Advancements in the characterisation of oligonucleotides by high performance liquid chromatography‐mass spectrometry in 2021: A short review

Analytical Science Advances, 2022
The first oligonucleotide therapeutic was approved by the Food and Drug Administration in 1998, and since then, 12 nucleic acids have been commercialised as medicines.
Fabien Hannauer, Rachelle Black, Andrew D. Ray, Eugen Stulz, G. John Langley, Stephen W. Holman   +5 more
doaj   +1 more source

Easy Computation of the Various Topologies and Modes of Liquid–Liquid Partition Chromatography by the Theory of Random Walks

Separations, 2021
The article revisits the discrete recurrence method to model the instruments of liquid–liquid partition chromatography as counter-current chromatography (CCC) and centrifugal partition chromatography (CPC).
Frédéric R. Dijoux, Tsvetelina Mandova
doaj   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source