Results 101 to 110 of about 92,178 (328)

Surviving a Genome Collision: Genomic Signatures of Allopolyploidization in the Recent Crop Species [PDF]

, 2017
Polyploidization has played a major role in crop plant evolution, leading to advantageous traits that have been selected by humans. Here, we describe restructuring patterns in the genome of Brassica napus L., a recent allopolyploid species.
Chalhoub, Boulos, Samans, Birgit, Snowdon, Rod J.   +2 more
core   +1 more source

Reference‐Guided Chromosome‐by‐Chromosome de novo Assembly at Scale Using Low‐Coverage High‐Fidelity Long‐Reads with HiFiCCL

Advanced Science, EarlyView.
HiFiCCL, as the first assembly framework specifically designed for low‐coverage high‐fidelity reads, improves the assembly quality of existing assemblers and also enhances downstream applications such as large structural variant (SV) detection (>10 000 bp), synteny analysis, pangenome graph construction, and graph‐based individual‐specific germline SVs
Zhongjun Jiang, Weihua Pan, Runtian Gao, Heng Hu, Wentao Gao, Murong Zhou, Yu‐Hang Yin, Zhipeng Qian, Shuilin Jin, Guohua Wang   +9 more
wiley   +1 more source

Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridization [PDF]

, 1990
A new strategy for analyzing chromosomal evolution in primates is presented using chromosomal in situ suppression (CISS) hybridization. Biotin-labeled DNA libraries from flow-sorted human chromosomes are hybridized to chromosome preparations of ...
Anna Jauch, Bickmore, Cremer, Cremer, de Grouchy, Dutrillaux, Holmquist, Johannes Wienberg, Lalley, Lichter, Lichter, Lichter, O'Brien, Ohno, Pinkel, Roscoe Stanyon, Seuánez, Stanyon, Thomas Cremer, Van Dilla, Weber, Wienberg, Yunis   +22 more
core   +1 more source

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

Advanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son, Goeun Kim, Jungjoon K. Lee   +2 more
wiley   +1 more source

A genetic network that suppresses genome rearrangements in Saccharomyces cerevisiae and contains defects in cancers. [PDF]

, 2016
Gross chromosomal rearrangements (GCRs) play an important role in human diseases, including cancer. The identity of all Genome Instability Suppressing (GIS) genes is not currently known.
Bell, Sara N, Clotfelter, Sarah P, de Souza, Jorge ES, de Souza, Sandro J, Fonseca, André F, Kolodner, Richard D, Martinez, Sandra L, Nene, Rahul V, Putnam, Christopher D, Somach, Steven B, Srivatsan, Anjana   +10 more
core   +2 more sources

Mechanisms of Aristolochic Acid Resistance in Specialist Butterflies and Evolutionary Insights for Potential Protective Pathways

Advanced Science, EarlyView.
The study provides an extreme example of insect adaptation to highly toxic defenses of host plants, and investigates the complex strategies to resist carcinogenic aristolochic acids, including physical isolation, metabolic detoxification, and DNA repair.
Yang Luan, Yubo Zhang, Jingjing Li, Jianqing Zhu, Yuyang Lei, Yushi Hu, Zhenqiang Xin, Tianpei Xie, Jiang Zheng, Yuanyuan Lin, Jingjing Shen, Yiyi Cao, Xinyue You, Jing Xi, Jiaying Wu, Weiying Liu, Xinyu Zhang, Yuanting Zheng, David J. Lohman, Leming Shi, Wei Zhang   +20 more
wiley   +1 more source

Failure of NIPT to detect constitutional chromoanasynthesis involving chromosome 21 in a case of fetal hydrops—A case report

Clinical Case Reports, 2019
We report a case of a de novo ring 21 complex chromosomal rearrangement in a fetus presenting with hydrops. Noninvasive prenatal testing (NIPT) failed to detect the imbalance.
Kathleen Bone, Melissa Jean MacPherson, Judy Chernos, Julie Lauzon   +3 more
doaj   +1 more source

Inferring processes underlying B-cell repertoire diversity

, 2015
We quantify the VDJ recombination and somatic hypermutation processes in human B-cells using probabilistic inference methods on high-throughput DNA sequence repertoires of human B-cell receptor heavy chains.
Aleksandra M. Walczak, Curtis G. Callan, Dunn-Walters DK, Janeway C, Quentin Marcou, Shapiro GS, Shiokawa S, Stryer L, Thierry Mora, Yuval Elhanati, Zachary Sethna   +10 more
core   +1 more source

On a break with the X: the role of repair of double-stranded DNA breaks in X-linked disease [PDF]

, 2012
The problem of managing free reactive DNA ends in eukaryotic cells has resulted in the development of a number of mechanisms in order to ensure that free ends are rendered non-reactive, or that the double-strand DNA breaks generating the free ends are ...
Cecceroni, Lucia, De Caris, Laura, Tummala, Hemanth   +2 more
core   +4 more sources

Time‐Efficient and Informatic‐Skill‐Light Gap‐Filling for Telomere‐to‐Telomere Genome Assembly

Advanced Science, EarlyView.
The paper introduces a novel auxiliary software toolbox GapSuite, consisting of two tools Gap‐Aid and Gap‐Graph, which guides users to fill gaps in chromosome‐level genome assembly using sequence‐extension‐based and assembly‐graph‐based strategies. The two tools enable users with limited informatics expertise to efficiently complete gap‐filling on ...
Dong Xu, Xianjia Zhao, Lianguang Shang, Shaolong Tian, Yanchun Li, Huaming Wen, Qiang Xu, Dongxi Li, Weihua Pan   +8 more
wiley   +1 more source