Results 91 to 100 of about 699,106 (307)

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

(Ovo-)viviparity in the darkling beetle, Alegoria castelnaui (Tenebrioninae: Ulomini), from Guadeloupe

European Journal of Entomology, 2010
In the course of cytogenetic studies on Alegoria castelnaui Fleutiaux & Sallé 1889 (Coleoptera: Tenebrionidae: Tenebrioninae: Ulomini) from Guadeloupe, a number of adult specimens were dissected.
Anne Marie DUTRILLAUX, Dominique PLUOT-SIGWALT, Bernard DUTRILLAUX   +2 more
doaj   +1 more source

Immunocytochemical localization of chromatin regions UV-microirradiated in S phase or anaphase : Evidence for a territorial organization of chromosomes during cell cycle of cultured Chinese hamster cells [PDF]

, 1983
Chinese hamster cells (M3-1 line) in S phase were laser-UV-microirradiated (λ, 257 nm) at a small site of the nucleus. Cells were fixed either immediately thereafter or in subsequent stages of the cell cycle, including prophase and metaphase.
Avivi, Bennett, Berns, Boveri, Boveri, Comings, Cornelis, Cornelis, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Earnshaw, Gray, Hager, Hancock, Hancock, Hens, Laemmli, Rabl, Regan, Sakharov, Sedat, Sperling, Stack, Trosko, Vogel, Zorn   +33 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Chromosome Level Genome Assembly and Comparative Genomics between Three Falcon Species Reveals an Unusual Pattern of Genome Organisation

Diversity, 2018
Whole genome assemblies are crucial for understanding a wide range of aspects of falcon biology, including morphology, ecology, and physiology, and are thus essential for their care and conservation.
Sunitha Joseph, Rebecca E. O’Connor, Abdullah F. Al Mutery, Mick Watson, Denis M. Larkin, Darren K. Griffin   +5 more
doaj   +1 more source

Karyotype evolution in progress: A new diploid number in Belostoma candidulum (Heteroptera: Belostomatidae) from Argentina leading to new insights into its ecology and evolution [PDF]

, 2014
A novel chromosome complement (2n = 14 = 12 + XY/XX; male/female sex chromosomes), male meiosis behaviour, heterochromatin characterization, and frequency and distribution of chiasmata are described for the first time in specimens from a natural ...
Bressa, Maria Jose, Chirino, Monica Gabriela   +1 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Chromosomal control of non-gliadin proteins from the 70% ethanol extract of wheat endosperm

, 1975
The non-gliadin fraction of the 70% ethanol extracts of compensated nulli-tetrasomics and ditelosomics of Triticum aestivum cv. Chinese Spring has been analyzed by combined electrofocusing and electrophoresis.
A. Bozzini, B. L. Johnson, B. L. Johnson, C. Aragoncillo, C. W. Wrigley, C. W. Wrigley, F. Garcia-Olmedo, F. García-Olmedo, J. D. Eastin, K. A. Siddiqui, K. Noda, K. W. Shepherd, M. A. Rodriguez Loperena, Pilar Carbonero, R. A. Orth, R. Riley, R. Solari, R. Solari, W. J. R. Boyd, W. J. R. Boyd   +19 more
core   +1 more source

Comparative chromosome band mapping in primates byin situ suppression hybridization of band specific DNA microlibraries [PDF]

, 1991
A DNA-library established from microdissected bands 8q23 to 8q24.1 of normal human chromosomes 8 (Lüdecke et al., 1989) was used as a probe for chromosomal in situ suppression (CISS-) hybridization to metaphase chromosomes of man and primates including ...
A. Jauch, B. Dutrillaux, B. Horsthemke, C. Lengauer, C. Lengauer, D. Pinkel, D. Pinkel, H. J. Lüdecke, H.J. Lüdecke, J. Wienberg, J. Wienberg, J.J. Yunis, M. Klever, M. Small, P. Lichter, P.A. Lalley, R. Stanyon, R. Stanyon, R. Stanyon, S.J. O'Brien, T. Cremer   +20 more
core   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source