In vitro amplification of whole large plasmids via transposon-mediated oriC insertion
BioTechniques, 2021 DNA amplification is a fundamental technique in molecular biology. The replication cycle reaction is a new method for amplification of large circular DNA having oriC sequences, which is a replication initiation site of the Escherichia coli chromosome. We
Seia Nara, Masayuki Su'etsugu
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Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [PDF]
PeerJ, 2022 Background Cyclocytidine hydrochloride (HCl) has been reported to inhibit DNA synthesis by affecting DNA polymerase. Here, we tested the antiviral effect of cyclocytidine on hepatitis B virus (HBV) DNA synthesis, which is reliant on DNA polymerase ...
Xue Wang +7 more
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Serious overestimation in quantitative PCR by circular (supercoiled) plasmid standard: microalgal pcna as the model gene. [PDF]
PLoS ONE, 2010 Quantitative real-time PCR (qPCR) has become a gold standard for the quantification of nucleic acids and microorganism abundances, in which plasmid DNA carrying the target genes are most commonly used as the standard.
Yubo Hou +3 more
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Extrachromosomal circular DNA: a new potential role in cancer progression
Journal of Translational Medicine, 2021 Extrachromosomal circular DNA (eccDNA) is considered a circular DNA molecule that exists widely in nature and is independent of conventional chromosomes. eccDNA can be divided into small polydispersed circular DNA (spcDNA), telomeric circles (t-circles),
Tianyi Wang +3 more
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TeCD: The eccDNA Collection Database for extrachromosomal circular DNA
BMC Genomics, 2023 Background Extrachromosomal circular DNA (eccDNA) is a kind of DNA that widely exists in eukaryotic cells. Studies in recent years have shown that eccDNA is often enriched during tumors and aging, and participates in the development of cell physiological
Jing Guo +4 more
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Journal of Hematology & Oncology, 2020 Extrachromosomal circular DNA was recently found to be particularly abundant in multiple human cancer cells, although its frequency varies among different tumor types.
Yuanliang Yan +7 more
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Hepatitis B virus cccDNA is formed through distinct repair processes of each strand
Nature Communications, 2021 HBV covalently closed circular DNA (cccDNA) enables and persists in chronic infection, but the molecular mechanism of its formation is unclear. Here, Wei and Ploss elucidate the detailed kinetics and biochemical steps by which the relaxed circular DNA is
Lei Wei, Alexander Ploss
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Deciphering extrachromosomal circular DNA in Arabidopsis
Computational and Structural Biotechnology Journal, 2021 Extrachromosomal circular DNA (eccDNA) is independent of the chromosome and exists in many eukaryotes. However, the nature and origin of eccDNA in plants remains unclear.
Keyi Wang +15 more
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Characterization of Extrachromosomal Circular DNA in Primary and Cisplatin-Resistant High-Grade Serous Ovarian Cancer. [PDF]
Genes (Basel)Wang Y +8 more
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Circular Dichroism of DNA [PDF]
European Journal of Biochemistry, 1970 The circular dichroism of sixteen DNA samples from fourteen species has been determined. Plots of molar ellipticity against base composition show considerable scatter, which is much too great to be accounted for in terms of reproducibility of materials and measurements.
W B, Gratzer, L R, Hill, R J, Owen
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