Results 241 to 250 of about 1,753,818 (296)

Real‐Time Monitoring of In Vitro Transcription for Production of mRNA Using Raman Spectroscopy

open access: yesBiotechnology and Bioengineering, EarlyView.
Schematic depicting the workflow for model building and validation using Raman spectroscopy for real‐time monitoring of IVT. ABSTRACT The success of COVID‐19 mRNA vaccines showcased the transformative potential of this adaptable, plug‐and‐play, and rapid response platform.
Julia Puppin Chaves Fulber   +4 more
wiley   +1 more source

Engineering of CoA‐Acylating Butyraldehyde Dehydrogenase for Enhanced 1,3‐Butanediol Production in Escherichia coli

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Microbial production of 1,3‐butanediol (1,3‐BDO) offers a renewable route to this versatile C4 chemical. However, the low performance of CoA‐acylating butyraldehyde dehydrogenase (Bld), which contains a catalytic cysteine, limits efficient production in recombinant Escherichia coli (E. coli).
Seunghyun Cho   +5 more
wiley   +1 more source

Impact of Process Interruptions in the Production of Lysates for Cell‐Free Expression Systems

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free gene expression systems offer cell‐like functionalities outside the confines of the cell, garnering increasing interest for applications from biomanufacturing to sensing. As applications expand, the need to implement economically scaled processes to produce cellular lysates grows.
Katherine A. Rhea   +11 more
wiley   +1 more source

Genetically Programmed Control of Overflow Metabolism

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Overflow metabolism is a highly undesirable phenomenon that occurs in virtually all cell factories. Although several schemes have been applied to avoid overflow metabolism, this problem remains far from being solved. In this study, a genetic sensor for detecting overflow metabolism was designed, and its substrate specificity and reversibility ...
Lærke M. Jensen   +3 more
wiley   +1 more source
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Civil engineering

2021
George Steele, George Steele
openaire   +2 more sources

Nanotechnology in Civil Engineering

Advances in Structural Engineering, 2004
The transcendent technologies, which are the primary drivers of the twenty first century and the new economy, include nanotechnology, microelectronics, information technology and biotechnology as well as the enabling and supporting civil infrastructure systems and materials.
openaire   +1 more source

The Engineer and Civilization

Science, 1925
To sum up the whole matter, the engineer, either as an individual or as a collective type, is simply a link in the chain of human progress—a chain the links of which, in one form or another, run back into a past removed from our own time by tens of thousands of years, to go to no higher figures.
openaire   +2 more sources

Civil Engineering

2023
Xiaowei Wang, Yue Li
openaire   +1 more source

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