Results 221 to 230 of about 1,316,499 (288)

Engineered Bacterial Ligand From Streptococcus pneumoniae on Macroporous Resins for Selective Affinity Capture of Secretory IgA

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Secretory immunoglobulin A (sIgA) is a promising emerging biopharmaceutical candidate, but it currently lacks a standardized platform for purification. To address this, a novel affinity chromatography resin was developed by immobilizing protein variants of the Streptococcus pneumoniae surface protein, SpsA, as an affinity ligand.
David Scheich, Andres Männik, Oliver Meikar, Joan Teyra, Laura Kibena, Sebastiaan Eeltink, Rainer Hahn, Alois Jungbauer, Nico Lingg   +8 more
wiley   +1 more source

Evaluating AI-powered learning assistants in engineering higher education with implications for student engagement, ethics, and policy. [PDF]

Sci Rep
Sajja R, Sermet Y, Fodale B, Demir I.
europepmc   +1 more source

Real‐Time Monitoring of In Vitro Transcription for Production of mRNA Using Raman Spectroscopy

Biotechnology and Bioengineering, EarlyView.
Schematic depicting the workflow for model building and validation using Raman spectroscopy for real‐time monitoring of IVT. ABSTRACT The success of COVID‐19 mRNA vaccines showcased the transformative potential of this adaptable, plug‐and‐play, and rapid response platform.
Julia Puppin Chaves Fulber, Xingge Xu, Hadi El Radi, Kevin Grollier, Amine A. Kamen   +4 more
wiley   +1 more source

Macro-micro deformation characteristics of undisturbed loess under different hydraulic paths. [PDF]

Sci Rep
Fu W, Shao S, She F, Luo A, Zhao Z.
europepmc   +1 more source

Engineering of CoA‐Acylating Butyraldehyde Dehydrogenase for Enhanced 1,3‐Butanediol Production in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Microbial production of 1,3‐butanediol (1,3‐BDO) offers a renewable route to this versatile C4 chemical. However, the low performance of CoA‐acylating butyraldehyde dehydrogenase (Bld), which contains a catalytic cysteine, limits efficient production in recombinant Escherichia coli (E. coli).
Seunghyun Cho, Tayyab Islam, Sobia Islam, Junhak Lee, Sung Won Jung, Sunghoon Park   +5 more
wiley   +1 more source

Shear behavior of high-strength reinforced concrete beams with circular openings under fire exposure. [PDF]

Sci Rep
Sedawy AE, Beshr AAA, Mahmoud IA.
europepmc   +1 more source

Impact of Process Interruptions in the Production of Lysates for Cell‐Free Expression Systems

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free gene expression systems offer cell‐like functionalities outside the confines of the cell, garnering increasing interest for applications from biomanufacturing to sensing. As applications expand, the need to implement economically scaled processes to produce cellular lysates grows.
Katherine A. Rhea, Ethan N. Walters, Julie L. Zacharko, Mia J. Seergae, Thomas R. Laws, Thomas C. Maishman, Yun‐Nam Choi, John T. Lazar, Ashty Karim, Weston Kightlinger, Michael C. Jewett, Matthew W. Lux   +11 more
wiley   +1 more source

Physical model study on the mechanism of floor heave for the deep-buried roadway excavated in soft rock of gently inclined thin strata. [PDF]

Sci Rep
Chen F, Wang E, Miao C, Sun X, Song P, Zhao C.   +5 more
europepmc   +1 more source

Genetically Programmed Control of Overflow Metabolism

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Overflow metabolism is a highly undesirable phenomenon that occurs in virtually all cell factories. Although several schemes have been applied to avoid overflow metabolism, this problem remains far from being solved. In this study, a genetic sensor for detecting overflow metabolism was designed, and its substrate specificity and reversibility ...
Lærke M. Jensen, Andriy Glushchuk, Thilde P. Hjorth, Alvaro R. Lara   +3 more
wiley   +1 more source

Culvert Retrofit with Green Filter Media for the Removal of Phosphorus from Stormwater Runoff. [PDF]

Materials (Basel)
Bagchi S, Zhang Z, Olayiwola O, Mandala B, Datta R, Giri S, Lathrop R, Sarkar D.   +7 more
europepmc   +1 more source