SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Effects of ascorbic acid on intestinal flora and metabolites of C57 mice exposed to formaldehyde in digestive tract. [PDF]
Ling X +6 more
europepmc +1 more source
Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley +1 more source
Rapid reshaping of the soil microbiome and metabolome during short-term flooding and draining in rice. [PDF]
Lu Y +5 more
europepmc +1 more source
The Gegenbauer Polynomial technique: the evaluation of a class of Feynman diagrams [PDF]
A. V. Kotikov
openalex +1 more source
What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman +4 more
wiley +1 more source
Enhancing Ishihara and educational images using machine learning: toward accessible learning for colorblind individuals. [PDF]
Prajapati AR, Goyal A.
europepmc +1 more source
Event-Driven Inconsistency Detection Between UML Class and Sequence Diagrams [PDF]
Lazzari, Luan, Farias, Kleinner
openalex
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima +4 more
wiley +1 more source
Automated inspection of P&ID object recognition using deep learning. [PDF]
Kim JB, Moon Y, Han ST, Mun D.
europepmc +1 more source

