Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Three-Dimensional Morphometric Analysis of the Maxillary Sinus-Nasal Complex in Adolescents With Unilateral Cleft Lip and Palate Using Cone‑Beam Computed Tomography. [PDF]
Lam MKC +7 more
europepmc +1 more source
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
Improved cosmetic and functional outcomes with V-shaped mini flap and dermofat graft combination for revision of cleft lip scars: A retrospective observational study. [PDF]
Akkoc MF +7 more
europepmc +1 more source
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley +1 more source
Which intraoral parameters are prognostic factors for speech outcomes in incomplete cleft palate? [PDF]
An JH, Seo HJ, Lee CH, Park GW, Bae YC.
europepmc +1 more source
Small RNA pathways in mammalian oocytes
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
Secondary Revision With Boot Flap in a Case of Tessier No. 0 Cleft. [PDF]
Ueda K, Hirota Y, Kino H, Seo N, Oe M.
europepmc +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Management of Cubital Tunnel Syndrome in Split-Hand/Split-Foot Malformation: A Case Report and Review of Ulnar Nerve Dependence in Cleft Hand Prehension. [PDF]
Stidham S +3 more
europepmc +1 more source

