Results 61 to 70 of about 3,839 (265)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Moderatio and Clementia In Late Roman Republic And Early Principate
Classica, Revista Brasileira de Estudos Clássicos, 2023 This paper is concerned with the role played by clementia (mercy/ clemency) as a particular peacebuilding skill in Late Roman Republic and Early Principate.
Gilson Charles dos Santos
doaj
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
History of Science in South Asia, 2016 We present here a critical edition of the numerical tables of the Karaṇakesarī, an eclipse-computation table-text authored by Bhāskara in the latter half of the 17th century, and known to us from three manuscripts.
Anuj J. Misra +2 more
doaj +1 more source
FEBS Open Bio, EarlyView.TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
The Table Text Jagadbhūṣaṇa of Haridatta
History of Science in South AsiaIn the seventeenth century, the astronomer Haridatta of Mewar, Rājasthān, produced a table text named the Jagadbhūṣaṇa (epoch Śaka 1560, or 1638 CE). This table text provided calendar makers with a complete set of data and associated procedures for the ...
Keshav Melnad +2 more
doaj +1 more source
Zagłada Żydów, 2013 War crimes trials roused considerable resistance in Germany. Here the author analyzes opposition to the Malmédy Trial, conducted at Dachau in 1946, citing documents made available under the 1998 Nazi War Crimes Disclosure Act – in particular those of ...
Jerome S. Legge, Jr.
doaj +1 more source