Results 61 to 70 of about 3,073,351 (268)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

High Pan-Immune Inflammation Values are Associated with Prolonged Length of Hospital Stay in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease

International Journal of General Medicine
Yan Lu, Haoyang Guo, Qiaohong Zhang Clinical Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, People’s Republic of ChinaCorrespondence: Yan Lu, Clinical Laboratory, Affiliated Dongyang Hospital of Wenzhou ...
Lu Y, Guo H, Zhang Q
doaj  

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Needs Analysis of Supportive Care for Postoperative Wound Rehabilitation in Anal Fistula Patients Based on the Kano Model

Journal of Multidisciplinary Healthcare
Chunxia Zhou,1 Yao He,1 Peirong Yang,1 Jie Du,2 Chuanhua Yang,1 Xue Wang1 1Department of General Surgery, the Fourth West China Hospital of Sichuan University, Chengdu City, People’s Republic of China; 2Department of Female Pelvic Medicine and ...
Zhou C, He Y, Yang P, Du J, Yang C, Wang X   +5 more
doaj  

The Experience of the Fight Against COVID-19 in Clinical Laboratory Departments from Chengdu, China

Risk Management and Healthcare Policy, 2021
Qin Wang,* Chenggui Liu,* Jian Xu, Peipei Song, Shuzhe Yang, Qin Zhang Department of Clinical Laboratory, Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu ...
Wang Q, Liu C, Xu J, Song P, Yang S, Zhang Q   +5 more
doaj  

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Development and Evaluation of a Handmade Lateral Flow Immunoassay for the Rapid Detection of Carbapenem-producing Gram-Negative Bacteria in China

Infection and Drug Resistance
Cai-Yun Zhang,1,* Jun Ji,2,* Xiu-Zhen Chen,3,4 Wei-Xiao Wang,3,4 Wei Chen,3,4 Xiao-Li Cao2 1The key Laboratory of Hepatology of Nanjing, the Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine, Nanjing ...
Zhang CY, Ji J, Chen XZ, Wang WX, Chen W, Cao XL   +5 more
doaj  

Detection and management of differences in biochemical results across identical analyzers in high-volume laboratories: a practical internal quality control-based method

Journal of Laboratory Medicine
Ensuring result comparability across identical analyzers is critical in high-throughput clinical laboratories. ISO 15189:2022 emphasizes this requirement (clause 7.3.7.4), particularly for assays with low within-subject biological variation (BV), where ...
Doruk Özlem Gürsoy, Uysal Sezer, Gözgöz Hazar, Gündoğdu Burak, Örmen Murat, Tuncel Pınar, Çoker Canan   +6 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Association Between Systemic Immune-Inflammation Index in Early Pregnancy and Preeclampsia: A Multicenter Cohort Study

Journal of Inflammation Research
Qiong Li,1,* Meng Li,2,* Chenyang Zhao,1 Xiaohu Chen,3 Chunyi Zhang,3 Ying Li,4 Yu Chen,4 Chaoyan Yue3 1Department of Obstetrics and Gynecology, The First People’s Hospital of Chenzhou, Chenzhou, People’s Republic of China; 2Department of
Li Q, Li M, Zhao C, Chen X, Zhang C, Li Y, Chen Y, Yue C   +7 more
doaj