Results 31 to 40 of about 2,207,856 (261)

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source

Optimizing diagnostic and management pathways for patients with eosinophilia of unknown origin: a multidisciplinary protocol for urgent and non-urgent evaluation

Frontiers in Medicine
IntroductionPersistent eosinophilia of unknown cause is a key feature of numerous health disorders. These conditions present diagnostic and management challenges, often leading to delayed treatment, increased morbidity and mortality and unnecessary ...
Silvia Sánchez-Ramón, Gloria Candelas Rodríguez, Jesús Gimeno Hernández, María Luisa González Gutiérrez, Sissy Fiorella Medina Salazar, Manuel Méndez Bailón, Begoña Navas Elorza, Ángel Nieto Sánchez, Teresa Robledo Echarren, Santiago Rueda Esteban, Marcos Oliver Fragiel Saavedra, María Dolores Zamora Barrios, Cristina Martínez Prada, Celina Benavente Cuesta, Celia Pinedo Sierra, EOS Multidisciplinary Group   +15 more
doaj   +1 more source

Measuring VLDL1 secretion in humans with an intravenous fat emulsion test

STAR Protocols, 2023
Summary: Tracer techniques to assess very-low-density lipoprotein (VLDL) secretion in humans are expensive, are time consuming, and require mathematical models to estimate VLDL kinetics. Here, we describe an alternative, time- and cost-efficient protocol
Matthäus Metz, Clemens Baumgartner, Herbert Stangl, Thomas Scherer   +3 more
doaj   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

A protocol to quantify cross-sectional and longitudinal differences in duction patterns

Frontiers in Neuroscience
Currently, there is no established system for quantifying patterns of ocular ductions. This poses challenges in tracking the onset and evolution of ocular motility disorders, as current clinical methodologies rely on subjective observations of individual
Kevin T. Willeford, Victoria Copel, Hua Rong   +2 more
doaj   +1 more source

A behavioral training protocol using visual perceptual learning to improve a visual skill

STAR Protocols, 2021
Summary: We describe a behavioral training protocol using visual perceptual learning (VPL) to improve visual detection skills in non-experts for subtle mammographic lesions indicative of breast cancer.
Sebastian M. Frank, Andrea Qi, Daniela Ravasio, Yuka Sasaki, Eric L. Rosen, Takeo Watanabe   +5 more
doaj   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization

STAR Protocols, 2022
Summary: Immunophenotyping of tumor-infiltrating lymphocytes (TILs) by flow cytometry can predict clinical efficacy of immunotherapy. However, several obstacles need to be overcome for developing a flow cytometry assay starting from solid tumor specimens.
Tamiyo Kobayashi, Shogo Kumagai, Rieko Doi, Elena Afonina, Shohei Koyama, Hiroyoshi Nishikawa   +5 more
doaj   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature

STAR Protocols, 2021
Summary: Current protocols for storage of white blood cells (WBCs) rely on constant refrigeration. The protocol described below explains the preparation of a fixative combination saline (FCS) formulation, which allows fixation of human WBCs and lysis of ...
Rajesh K. Chaurasia, Kapil B. Shirsath, Balvinder K. Sapra   +2 more
doaj   +1 more source