Results 91 to 100 of about 1,354,268 (327)

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

Research on timing method of prepayment meter based on edge computing

open access: yesDianzi Jishu Yingyong
With the construction of the power IoT and the increasing popularity of smart meters, solving the problem of abnormal clock of meters and correcting the clock deviation of meters has become an increasingly important task for power companies.
Li Jiangchao   +5 more
doaj   +1 more source

Multiphase clock-pulse generator uses simplified circuitry [PDF]

open access: yes, 1965
Multiphase clock-pulse generator converts a simple pulse train into nonoverlapping clock pulses.

core   +1 more source

An immune clock of human pregnancy

open access: yesScience immunology, 2017
High-dimensional analysis reveals the chronology of immunological adaptations during the course of human pregnancies. Following the hand of the immunological clock Immune function is altered during pregnancy to protect the fetus from an immunological ...
N. Aghaeepour   +25 more
semanticscholar   +1 more source

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

open access: yesFEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman   +3 more
wiley   +1 more source

Epigenetic age-predictor for mice based on three CpG sites

open access: yeseLife, 2018
Epigenetic clocks for mice were generated based on deep-sequencing analysis of the methylome. Here, we demonstrate that site-specific analysis of DNA methylation levels by pyrosequencing at only three CG dinucleotides (CpGs) in the genes Prima1, Hsf4 ...
Yang Han   +9 more
doaj   +1 more source

Gut Microbiota-Derived Short Chain Fatty Acids Induce Circadian Clock Entrainment in Mouse Peripheral Tissue

open access: yesScientific Reports, 2018
Microbiota-derived short-chain fatty acids (SCFAs) and organic acids produced by the fermentation of non-digestible fibre can communicate from the microbiome to host tissues and modulate homeostasis in mammals.
Y. Tahara   +9 more
semanticscholar   +1 more source

Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancer

open access: yesFEBS Letters, EarlyView.
Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello   +6 more
wiley   +1 more source

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