High‐Resolution Patterned Delivery of Chemical Signals From 3D‐Printed Picoliter Droplet Networks
3D‐printed picoliter droplet networks have been fabricated that control gene expression in bacterial populations by releasing chemical signals with precise spatial definition and high temporal resolution. This system of effector release is widely applicable, offering diverse applications in biology and medicine.
Jorin Riexinger+7 more
wiley +1 more source
Multimerized epitope tags for high-sensitivity protein detection. [PDF]
Stowers RS.
europepmc +1 more source
De Novo Design of Integrin α5β1 Modulating Proteins to Enhance Biomaterial Properties
NeoNectins are de novo‐designed miniproteins that selectively bind and stabilize the extended open form of integrin α5β1. When grafted onto biomaterials including hydrogel and titanium, they promote cell attachment and spreading in vitro and promoting tissue integration and bone growth in animal models, demonstrating broad potential for regenerative ...
Xinru Wang+25 more
wiley +1 more source
<u>Ad</u>vanced <u>v</u>iral genome <i><u>i</u>n vitro</i> <u>C</u>as9 <u>e</u>diting (AdVICE): an overnight method for traceless and limitless manipulation of adenoviral and vector genomes with large transgenes. [PDF]
Vergnes J-B, Roger B, Iggo R, Wodrich H.
europepmc +1 more source
Designing Physical Unclonable Functions From Optically Active Materials
Assigning unforgeable “fingerprints” to manufactured goods is a key strategy to fight global counterfeiting. Optical physical unclonable functions (PUFs) are chemically generated random patterns of optically active materials serving as unique authenticators.
Maxime Klausen+2 more
wiley +1 more source
DAPE cloning with modified primers for producing designated lengths of 3' single-stranded ends in PCR products. [PDF]
Lee S+5 more
europepmc +1 more source
Traceless Photopolymerization with Non‐Pulsed Red Light Enables 3D‐Printable Cell‐Laden Hydrogels
The paper presents a red‐light‐induced polymerization process, initiated by the FDA‐approved dye methylene blue and the cytocompatible co‐initiator triethanolamine, for fabricating hydrogels. This photopolymerization process is oxygen‐tolerant, cytocompatible, and, most importantly, leaves no color traces, resulting in completely transparent scaffolds.
Ali Eftekhari+10 more
wiley +1 more source
Identification and characterization of DFR gene family and cloning of candidate genes for anthocyanin biosynthesis in pepper (Capsicum annuum L.). [PDF]
An Y, Li N, Zhang R, Wang S, Wang J.
europepmc +1 more source