Results 291 to 300 of about 1,845,886 (394)

Protocol to quantify site-specific carbohydrate sulfation at immune checkpoint proteins in mammalian cells. [PDF]

STAR Protoc
Chou WC, Chen WT, Jou YS, Shen CY.
europepmc   +1 more source

Transcript Identification Using Arrayed Hydrogels With TrapFISH

Advanced Materials Technologies, EarlyView.
TrapFISH is a novel method for high‐throughput single‐cell transcriptomics. It embeds cells in hyaluronic acid hydrogel beads and uses probe hybridization to quantify custom gene panels. By arraying hydrogels in microfluidic devices, cell barcoding is removed, enhancing scalability for studying cellular heterogeneity and rare cell states by targeting ...
David B. Morse, Zenon Toprakcioglu, Akhila Denduluri‐Marthi, James D. Brenton, Craig J. Thomas, Tuomas P. J. Knowles   +5 more
wiley   +1 more source

'Two in One' Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion-Ligation and Screening of Positive Recombinants by Unaided Eyes. [PDF]

Curr Issues Mol Biol
Zhang X, Teng C, Lyu K, Lyu S, Fan Y.
europepmc   +1 more source

Facile Fabrication of Large‐Area Biofunctionalized Metallic Nanostructures for High Performance Affinity Plasmonic Biosensors

Advanced Materials Technologies, EarlyView.
A novel approach enables facile and reproducible fabrication of gold nanoparticle arrays with tunable near‐infrared plasmonic properties and enhanced sensing accuracy. Using advanced lithography and ion milling, combined with innovative surface chemistry, the approach minimizes ohmic losses and non‐specific sorption, improving biomolecular detection ...
Dario Cattozzo Mor, Adam Benjamin Plšek, Prasanth Asokan, Van‐Truc Vu, Gizem Aktug, Elena Giordani, Elena Ricciardi, Jiri Perutka, Kamil Önder, Ladislav Fekete, Zdeněk Hubička, Chun‐Jen Huang, Jakub Dostalek   +12 more
wiley   +1 more source

biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at the <i>ACTB</i> locus. [PDF]

Synth Biol (Oxf)
Pelosse M, Marcia M.
europepmc   +1 more source

Mechanical Interactions Impact the Functions of Immune Cells and Their Application in Immunoengineering

Advanced Therapeutics, EarlyView.
This review covers the mechanical forces experienced by immune cells through cell–cell and cell–extracellular matrix interactions and how these forces influence their receptors and functions. These relationships between forces and cellular functions can be exploited using engineering techniques to modify the physical properties of materials for novel ...
Yu‐Chang Chen, Nghi M. Tran, Kyle H. Vining   +2 more
wiley   +1 more source

Split-and-pool synthesis to generate scalable combinatorial oligonucleotide libraries on magnetic nanoparticles. [PDF]

STAR Protoc
Nguyen JVL, Malic L, Nassif C, Da Fonte D, Tabrizian M.   +4 more
europepmc   +1 more source

SMYD3 Promotes Immune Evasion in Clear Cell Renal Cell Carcinoma via SREBP1‐Mediated Transactivation of CD47

Advanced Science, EarlyView.
Cancer cell‐intrinsic SMYD3 orchestrates an immunosuppressive microenvironment and impairs the response to PD‐1 blockade by reprogramming immune cells landscape in the tumor microenvironment of clear cell renal cell carcinoma (ccRCC). The immunomodulatory function of SMYD3 is mediated via the SREBP1/CD47 pathway.
Zhengfang Liu, Xiumei Zhao, Maolin Zang, Huiyang Yuan, Xin Qin, Xiaofeng Li, Shuo Zhao, Ruirong Tan, Keqiang Yan, Li Liu, Yidong Fan, Ning Zhang, Benkang Shi, Bo Han, Shouzhen Chen   +14 more
wiley   +1 more source

Protocol to study the effects of mutations near splicing sites on pre-mRNA splicing. [PDF]

STAR Protoc
Xie D, Peng Q, Tian Y, Han Y, Lu G.
europepmc   +1 more source

Fibronectin Fibers Progressively Lose Their Tension in Invasive Human Breast Carcinoma while Being Tensed in DCIS and Healthy Breast Tissue

Advanced Science, EarlyView.
Extracellular matrix remodeling is crucial in cancer progression. Using a peptide to probe the tension of ECM fibers, it was found that Fibronectin fibers gradually lose tension as human breast carcinoma progresses, while their tension is maintained in ductal carcinoma in situ (DCIS), the non‐invasive precursor of breast cancer. This loss of tension is
Arnaud Miéville, Charlotte M. Fonta, Cornelia Leo, Lucine Christe, Jörg Goldhahn, Gad Singer, Viola Vogel   +6 more
wiley   +1 more source