Results 161 to 170 of about 3,155,909 (302)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

When a Cluster Is a Cluster

Qeios
The study of epidemic spread has generally relied on the description of certain number of cases of an infectious diseases like COVID-19 in relation to time occurrence of disease manifestations rather than to the exact place of occurrence. In recent times, computer generated dot maps have facilitated the modeling of the spread of infectious epidemic ...
openaire   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Defining metabolic niches for marine microbial heterotrophs. [PDF]

Sci Adv
Reynolds RC, Weiss ACB, James CC, Kojima CY, Weissman JL, Thrash JC, Levine NM.   +6 more
europepmc   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Lifestyle-associated variation in type IV secretion systems between phytopathogenic and environmental <i>Ralstonia</i>. [PDF]

Microb Genom
Cowell TC, Cope-Arguello ML, Shin GY, Bogdanove AJ, Carpenter SCD, Nahar K, Saha A, Lowe-Power TM.   +7 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Key Factors and Evaluation Model of Valuable Metal Separation in Low-Concentration Smelting Slag

ACS Omega
Zhuandi Shao, Tiantian Wei, Xiujuan Zhang, Kang Liao, Xiaogang Hou, Hong Deng, Xueming Liu, Zhang Lin, Liyuan Chai   +8 more
doaj   +1 more source

High altitude-mediated immune remodeling accelerates aging. [PDF]

Sci Adv
Xiao Y, Li Y, Mao Y, Jin Z, Sun M, Wang X, Zhao G, Song Y, Li J, Zhang Z, Liu L, Feng S, Liu M, Chen Z, Guo X, Yu R, Li H, Yang C, Luo C, Li J, Zhao Z, Zhang J, Cai L, Ma H, Li S, Li S, Xu F, Xin G, Jiang Q, Huang F, Kang L, Zhang C.   +31 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source