Results 11 to 20 of about 374 (110)

Deep neural networks to register and annotate cells in moving and deforming nervous systems [PDF]

eLife
Aligning and annotating the heterogeneous cell types that make up complex cellular tissues remains a major challenge in the analysis of biomedical imaging data.
Adam A Atanas, Alicia Kun-Yang Lu, Brian Goodell, Jungsoo Kim, Saba N Baskoylu, Di Kang, Talya S Kramer, Eric Bueno, Flossie K Wan, Karen L Cunningham, Brandon Weissbourd, Steven W Flavell   +11 more
doaj   +2 more sources

Comparison of activities of transcription factor NF-κB from two jellyfish models [PDF]

Comparative Immunology Reports
Herein, transcription factor Nuclear Factor-kappaB (NF-κB) is characterized as the downstream effector of putative innate immune signaling pathways from two model jellyfish, Aurelia aurita (Aa) and Clytia hemisphaerica (Ch). Both jellyfish NF-κB proteins
Leah M. Williams, Wei Wang, Alexandra V. Grigoryeva, Alejandra Navarro-Rosado, Jada A. Peart, Angela Calderon, Catherine L. Gill, Susan Black, Kristina M. Alsante, Aidan T. Lackstrom, Molecular Biology Laboratory, Brandon Weissbourd, Mengrui Wang, Christopher J. DiRusso, Lianne B. Cohen, Zeba Wunderlich, Brian P. Grone, Thomas D. Gilmore   +17 more
doaj   +2 more sources

Spatiotemporal variations of zooplankton community structure in the oyster (Crassostrea gigas)-macroalgae reef dual ecosystems adjacent to Luanhe River Estuary. [PDF]

PLoS ONE
Majority of macrozooplankton have a wider dietary niche breadth and utilize small invertebrates, microzooplankton and mesozooplankton, so effect on primary production might be through trophic cascading effect. To better understand the ecosystem structure
Min Xu, Qi Zhao, Shenzhi Wang, Yun Wang, Jiabin Shen, Yi Zhang, Linlin Yang, Kaida Xu, Xiaolong Hou, Yunling Zhang, Haipeng Zhang, Takayoshi Otaki, Teruhisa Komatsu, Yufu Xu   +13 more
doaj   +2 more sources

First person – Marion Lechable and Alexandre Jan [PDF]

Biology Open, 2020
First Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers.
doaj   +2 more sources

Conserved meiotic mechanisms in the cnidarian Clytia hemisphaerica revealed by Spo11 knockout. [PDF]

Sci Adv, 2023
AbstractDuring meiosis, DNA recombination allows the shuffling of genetic information between the maternal and paternal chromosomes. Recombination is initiated by double strand breaks (DSBs) catalyzed by the conserved enzyme Spo11. How this crucial event is connected to other meiotic processes is surprisingly variable depending on the species. Here, we
Munro C, Cadis H, Pagnotta S, Houliston E, Huynh JR.   +4 more
europepmc   +6 more sources

Past, present and future of Clytia hemisphaerica as a laboratory jellyfish [PDF]

, 2022
The hydrozoan species Clytia hemisphaerica was selected in the mid-2000s to address the cellular and molecular basis of body axis specification in a cnidarian, providing a reliable daily source of gametes and building on a rich foundation of experimental embryology.
Evelyn, Houliston, Lucas, Leclère, Catriona, Munro, Richard R, Copley, Tsuyoshi, Momose   +4 more
openaire   +4 more sources

High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion. [PDF]

Sci Rep, 2018
AbstractTargeted mutagenesis using CRISPR/Cas9 technology has been shown to be a powerful approach to examine gene function in diverse metazoan species. One common drawback is that mixed genotypes, and thus variable phenotypes, arise in the F0 generation because incorrect DNA repair produces different mutations amongst cells of the developing embryo ...
Momose T, De Cian A, Shiba K, Inaba K, Giovannangeli C, Concordet JP.   +5 more
europepmc   +5 more sources

In vivo imaging of epithelial wound healing in the cnidarian Clytia hemisphaerica demonstrates early evolution of purse string and cell crawling closure mechanisms. [PDF]

BMC Dev Biol, 2017
All animals have mechanisms for healing damage to the epithelial sheets that cover the body and line internal cavities. Epithelial wounds heal either by cells crawling over the wound gap, by contraction of a super-cellular actin cable ("purse string") that surrounds the wound, or some combination of the two mechanisms.
Kamran Z, Zellner K, Kyriazes H, Kraus CM, Reynier JB, Malamy JE.   +5 more
europepmc   +4 more sources

Spineless and overlooked: DNA metabarcoding of autonomous reef monitoring structures reveals intra‐ and interspecific genetic diversity in Mediterranean invertebrates

Molecular Ecology Resources, Volume 23, Issue 7, Page 1689-1705, October 2023., 2023
Abstract The ability to gather genetic information using DNA metabarcoding of bulk samples obtained directly from the environment is crucial to determine biodiversity baselines and understand population dynamics in the marine realm. While DNA metabarcoding is effective in evaluating biodiversity at community level, genetic patterns within species are ...
Anna Thomasdotter, Peter Shum, Francesco Mugnai, Marina Vingiani, Vincent Dubut, Florent Marschal, Marco Abbiati, Anne Chenuil, Federica Costantini   +8 more
wiley   +1 more source

Identification of an ERN1 target site within EGFP mRNA

Journal of Cellular Biochemistry, Volume 123, Issue 8, Page 1298-1305, August 2022., 2022
Abstract EGFP (enhanced green fluorescent protein) is one of the most common tools used in life sciences, including research focusing on proteostasis. Here we report that ERN1 (endoplasmic reticulum to nucleus signaling 1), which is upregulated by UPR (unfolded protein response), targets an RNA hairpin loop motif in EGFP mRNA.
Marius W. Baeken, Yohei Yokobayashi
wiley   +1 more source