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Cobra venom factor: Improved method for purification and biochemical characterization

Journal of Immunological Methods, 1984
A method to purify cobra venom factor (CVF) from cobra venom by sequential column chromatography is described which yields a product virtually free of phospholipase A2, a common contaminant of CVF preparations. The separation of phospholipase A2 from CVF was achieved by chromatography on Cibacron blue-agarose, a resin that tightly binds cobra venom ...
C W Vogel   +2 more
exaly   +3 more sources

Simple method for purification of phospholipase A from cobra venom

Toxicon, 1969
Abstract A simple method is described for the purification of phospholipase A. The enzyme preparation obtained was purified 20-fold, and was homogeneous on starch gel electrophoresis. The yield was 5 to 6 per cent with a recovery of 60 to 70 per cent of the total units present in the starting material.
B M, Braganca   +2 more
openaire   +2 more sources

Standardization of Cobra (Naja Flava) Venom Using the Graded Response Method

The Journal of Immunology, 1953
Summary The relationship between dosage of Naja flava venom and death-time of mice has been examined from experiments using intravenous injection. The observations were found to be well represented by the equation . From this formula, a function of death-time can be constructed that has a linear regression on the logarithm
P A, CHRISTENSEN, D J, FINNEY
openaire   +2 more sources

An investigation of the interaction of cobra venom cytotoxins with liposomes by the fluorescent-probe method

Chemistry of Natural Compounds, 1986
The interaction of cytotoxins Vc1, Vc5, and Vc6 of the venom of the Central Asian cobra with liposomes having a negative surface charge prepared from a mixture of phosphatidylcholine and palmitic acid (1:1, molar) has been investigated with the aid of a pyrene fluorescent probe. It has been shown that on interacting with liposomes a cytotoxin increases
T. F. Aripov   +2 more
openaire   +1 more source

Principles of the CoBRA Method

2012
The Cost Estimation, Benchmarking, and Risk Assessment (CoBRA) method combines multiple prediction approaches in that it aggregates techniques representing expert-based and data-driven estimation paradigms, within one hybrid estimation method. This chapter introduces the basic idea and terminology of the CoBRA method.
openaire   +1 more source

An improved method for the isolation from Naja naja venom of cobra factor (CoF) free of phospholipase A

Journal of Immunological Methods, 1979
An improved method is reported for the isolation from cobra (Naja naja) venom of cobra factor (CoF), the anticomplementary protein which is derived from cobra C3. Sequential chromatography on DEAE-Sepharose, Sephacryl-S200, and finally hydroxylapatite yielded 6.25 mg CoF per gram of crude venom.
Pepys, M B, Tompkins, C, Smith, A D
openaire   +2 more sources

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