Results 211 to 220 of about 167,532 (264)

A rapid colorimetric assay for DNA

Analytical Biochemistry, 1977
Abstract A rapid precise colorimetric method for the determination of DNA in the range of 25–1000 μg is reported. The method involves reacting DNA with diaminobenzoic acid · 2HCl (DABA) for 30 min at 60°C, followed by a determination of the optical density of the product at 420 nm. Perchloric acid extracts of DNA may be used in the assay.
F, Setaro, C D, Morley
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A colorimetric assay for immobilized chloroperoxidase

Canadian Journal of Microbiology, 1990
A rapid and sensitive colorimetric assay was developed for the estimation of chloroperoxidase activity. N,N,N′,N′-Tetramethyl-p-phenylenediamine was chosen from four potential chromogenic substrates because the blue product resulting from chloroperoxidase conversion gave the highest molar absorption.
T A, Kadima, M A, Pickard
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A Colorimetric Assay for Cytokinin Oxidase

Analytical Biochemistry, 1995
A simple and rapid colorimetric assay for cytokinin oxidase is described. The assay is based on the formation of a Schiff base between the enzymatic reaction product 3-methyl-2-butenal and p-aminophenol. The assay is effective in the submicromolar concentration range and can be used in crude plant extracts as well as in more highly purified ...
C A, Libreros-Minotta, P A, Tipton
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Colorimetric assay of catalase

Analytical Biochemistry, 1972
A simple colorimetric assay for catalase activity has been described using K2Cr2O7/acetic acid reagent. Kat. f values of different enzyme sources were determined by the colorimetric method and compared with the values obtained by titrimetric methods.
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Colorimetric protein assay techniques

Biotechnology and Applied Biochemistry, 1999
There has been an increase in the number of colorimetric assay techniques for the determination of protein concentration over the past 20 years. This has resulted in a perceived increase in sensitivity and accuracy with the advent of new techniques. The present review considers these advances with emphasis on the potential use of such technologies in ...
C V, Sapan, R L, Lundblad, N C, Price
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A quantitative colorimetric assay for semialdehydes

Analytical Biochemistry, 1990
We have studied the ability of the enzyme pyrroline-5-carboxylate dehydrogenase to oxidize aldehydes which are structurally similar to pyrroline-5-carboxylate, the enamine of gamma-glutamyl semialdehyde. These studies required the organic synthesis of semialdehydes not commercially available.
W C, Small, M E, Jones
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An ultrasensitive colorimetric assay for manganese

Analytical Biochemistry, 1988
An ultrasensitive colorimetric assay for manganese is described. It is based upon the catalysis, by Mn(II), of the photochemical oxidation of o-dianisidine, sensitized by riboflavin. Catalase increases the Mn(II)-catalyzed rate of photosensitized oxidation of dianisidine to the bisazobiphenyl, while superoxide dismutase inhibits the rate. The mechanism
W F, Beyer, I, Fridovich
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Colorimetric Assay for Acetaminophen in Serum

Therapeutic Drug Monitoring, 1984
A colorimetric method for determination of acetaminophen in serum, based on its reaction with 2-nitroso-1-naphthol-4-sulfonic acid, is described. The method is sensitive, rapid, and free from the interferences of serum matrix and most common drugs. The method can be performed manually or can be semiautomated. The results from this method correlate well
Z K, Shihabi, R M, David
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A quantitative colorimetric assay for squalene

Analytical Biochemistry, 1962
Abstract A rapid and sensitive quantitative colorimetric test for squalene has been devised. The color reaction involves the use of sulfuric acid and formaldehyde. The test will give a positive reaction with other compounds which contain double bonds and must consequently be used in conjunction with other methods which yield purified squalene.
G H, ROTHBLAT, D S, MARTAK, D, KRITCHEVSKY   +2 more
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Automated colorimetric assay of Norgestrel

Steroids, 1970
Abstract A relatively sensitive assay for the contraceptive steroid norgestrel, in pharmaceutical preparations, is described. The procedure involves the blue tetrazolium reaction. Optimum conditions for color development were established. The relative standard deviation for the assay, which has a sample rate of 30 hr−1, were of the order of 0.9% at a
P M, Short, C T, Rhodes
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