Results 251 to 260 of about 178,057 (307)
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Detection of opines by colorimetric assay
Analytical Biochemistry, 1987A colorimetric procedure for confirming the presence of arginine-derived opines (nopaline and octopine) in plant tissue extracts is described. Those materials are widely used as markers of plant cell transformation and tumorigenesis mediated by the tumor-inducing plasmids of Agrobacterium tumefaciens.
N S, Yang, S G, Platt, P, Christou
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An easy colorimetric assay for glycosyltransferases
Biochemistry (Moscow), 2010Glycosyltransferases are involved in biosynthesis of both protein-bound and non-bound glycans that have multiple and important biological functions in all species. A variety of methods for assaying glycosyltransferase activity have been developed driven by the specific interests and type of information required by researchers.
Rui, Shen +6 more
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Colorimetric assay for cellular transglutaminase
Analytical Biochemistry, 1989A colorimetric assay for cellular transglutaminase using 5-(biotinamido)pentylamine and polyvinylidine difluoride membranes for crude cellular preparations and purified enzyme has been developed. The biotinpentylamine substrate was incorporated into N,N-dimethylcasein by transglutaminase, the biotinylated products were adsorbed onto the membrane disks ...
W M, Jeon +4 more
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An improved colorimetric assay for polyols
Analytical Biochemistry, 1977Abstract A new assay for polyols was developed that minimizes interference by sugars. It is based on oxidation of alditols to formaldehyde by acidic periodate, reduction of the excess periodate with l -rhamnose, and a short-time determination of the formaldehyde with the Nash reagent. When glycerol was added to various crude biological materials and
S H, Bok, A L, Demain
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Colorimetric protein assay techniques
Biotechnology and Applied Biochemistry, 1999There has been an increase in the number of colorimetric assay techniques for the determination of protein concentration over the past 20 years. This has resulted in a perceived increase in sensitivity and accuracy with the advent of new techniques. The present review considers these advances with emphasis on the potential use of such technologies in ...
C V, Sapan, R L, Lundblad, N C, Price
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A quantitative colorimetric assay for semialdehydes
Analytical Biochemistry, 1990We have studied the ability of the enzyme pyrroline-5-carboxylate dehydrogenase to oxidize aldehydes which are structurally similar to pyrroline-5-carboxylate, the enamine of gamma-glutamyl semialdehyde. These studies required the organic synthesis of semialdehydes not commercially available.
W C, Small, M E, Jones
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A Colorimetric Assay for Cytokinin Oxidase
Analytical Biochemistry, 1995A simple and rapid colorimetric assay for cytokinin oxidase is described. The assay is based on the formation of a Schiff base between the enzymatic reaction product 3-methyl-2-butenal and p-aminophenol. The assay is effective in the submicromolar concentration range and can be used in crude plant extracts as well as in more highly purified ...
C A, Libreros-Minotta, P A, Tipton
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Colorimetric and titrimetric assay of isoniazid
Journal of Pharmaceutical and Biomedical Analysis, 1992Two methods are proposed for the determination of isoniazid in pure form or in tablets. In the first method chlorpromazine hydrochloride, when treated with 2-iodoxybenzoic acid as an oxidant in 50% w/v o-phosphoric acid solution, is oxidized to chlorpromazine free radical which absorbs at 530 nm.
A M, el-Brashy, S M, el-Ashry
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Colorimetric assay of catalase
Analytical Biochemistry, 1972A simple colorimetric assay for catalase activity has been described using K2Cr2O7/acetic acid reagent. Kat. f values of different enzyme sources were determined by the colorimetric method and compared with the values obtained by titrimetric methods.
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A rapid colorimetric assay for DNA
Analytical Biochemistry, 1977Abstract A rapid precise colorimetric method for the determination of DNA in the range of 25–1000 μg is reported. The method involves reacting DNA with diaminobenzoic acid · 2HCl (DABA) for 30 min at 60°C, followed by a determination of the optical density of the product at 420 nm. Perchloric acid extracts of DNA may be used in the assay.
F, Setaro, C D, Morley
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